LOCUS       sequence-153177-         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EGFP-tagged DmPAN3 in S2 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Braun JE, Huntzinger E, Fauser M, Izaurralde E
  TITLE     GW182 proteins directly recruit cytoplasmic deadenylase complexes to
            miRNA targets.
  JOURNAL   Mol Cell. 2011 Oct 7;44(1):120-33.
  PUBMED    21981923
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2011-10-7"; volume: "44(1)"; pages: "120-33"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmPAN3
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     38..55
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(78..95)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(105..146)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 ggatcctcga tcgaggctga tcagcgagct caggccttag aaggcacagt cgaggctgat
       61 cagcgggttt aaactcaatg gtgatggtga tgatgaccgg tacgcgtaga atcgagaccg
      121 aggagagggt tagggatagg cttaccttcg aaccgcgggc cctctagact cgagcggccg
      181 ctcagttggc cgcacttgac atcagttcgg agaaggcgtt ctccagacaa ttcttgagtt
      241 cagcatagga gacgatgagt accgattgct cgtcgcggga catcagttgc acgcgctcta
      301 tggagccagc atccagcttg ttgaggcatt gtacaatgtg tgcgtgatct agccagggcc
      361 ggccatcctc agtgacggaa tggaacaaat aatcacggaa taatttcaac atgtatctat
      421 caccagtctc agaccaagtg cagtccagat taaagtcggg tcgctcgttg atgctattaa
      481 gtttgaccaa aatgcgatat aaccgcccgt tctccatctc cttggccaac tcgtcctcct
      541 gcatatcaat tttgctctgc agtgcatcca gttgcgtata gaagcgggca ccaatcatcg
      601 gcatcaggtc ggtcacggag cgacgattat tggtggtgaa gaggtaaaca ataaagtttc
      661 gcagatcggt ggagtagttt cgcgtgacca tatcgatgct ggactggaca ttgtcgcgtt
      721 gcacggattg aagacagcgg caggccaaag ctaatactaa gcgacccaaa gcggtcagat
      781 cgtcctgctg atgcatatta accagcgcca agggattgga tgcattcgga tcgaattgcg
      841 ttatatccga aatgcagcat gagctaaacc gtacacgctt gcccgtcact ataatctttg
      901 tgggatccag aaccttgcag gcaagaccgg catggtggat ggcttttaag ccggcggtca
      961 gctgcatgat gatcgacca
//
