LOCUS       sequence-153178-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EGFP-tagged HseIF4E in Mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Peter D, Igreja C, Weber R, Wohlbold L, Weiler C, Ebertsch L, 
            Weichenrieder O, Izaurralde E
  TITLE     Molecular architecture of 4E-BP translational inhibitors bound to 
            eIF4E.
  JOURNAL   Mol Cell. 2015 Mar 19;57(6):1074-87. doi: 
            10.1016/j.molcel.2015.01.017. Epub 2015 Feb 19.
  PUBMED    25702871
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.01.017"; journalName: "Mol Cell"; date: 
            "2015-03-19- 19"; volume: "57"; issue: "6"; pages: "1074-87"
COMMENT     Sequence Label: pT7-EGFP-HseIF4E
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    843..964
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(880..899)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     934..953
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggacgagct gtacaagtcc ggactcagat ctcgagctca agcttcgaat tctgcaatgg
       61 cgactgtcga accggaaacc acccctactc ctaatccccc gactacagaa gaggagaaaa
      121 cggaatctaa tcaggaggtt gctaacccag aacactatat taaacatccc ctacagaaca
      181 gatgggcact ctggtttttt aaaaatgata aaagcaaaac ttggcaagca aacctgcggc
      241 tgatctccaa gtttgatact gttgaagact tttgggctct gtacaaccat atccagttgt
      301 ctagtaattt aatgcctggc tgtgactact cactttttaa ggatggtatt gagcctatgt
      361 gggaagatga gaaaaacaaa cggggaggac gatggctaat tacattgaac aaacagcaga
      421 gacgaagtga cctcgatcgc ttttggctag agacacttct gtgccttatt ggagaatctt
      481 ttgatgacta cagtgatgat gtatgtggcg ctgttgttaa tgttagagct aaaggtgata
      541 agatagcaat atggactact gaatgtgaaa acagagaagc tgttacacat atagggaggg
      601 tatacaagga aaggttagga cttcctccaa agatagtgat tggttatcag tcccacgcag
      661 acacagctac taagagcggc tccaccacta aaaataggtt tgttgtttaa ccgggatcca
      721 ccggatctag ataactgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
      781 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
      841 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
      901 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
      961 cttaacgcgt aaattgtaag cgttaatatt ttgttaaa
//
