LOCUS       sequence-153180-        1015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EGFP-tagged HseIF4E in Mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Peter D, Igreja C, Weber R, Wohlbold L, Weiler C, Ebertsch L, 
            Weichenrieder O, Izaurralde E
  TITLE     Molecular architecture of 4E-BP translational inhibitors bound to 
            eIF4E.
  JOURNAL   Mol Cell. 2015 Mar 19;57(6):1074-87. doi: 
            10.1016/j.molcel.2015.01.017. Epub 2015 Feb 19.
  PUBMED    25702871
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.01.017"; journalName: "Mol Cell"; date: 
            "2015-03-19- 19"; volume: "57"; issue: "6"; pages: "1074-87"
COMMENT     Sequence Label: pT7-EGFP-HseIF4E
FEATURES             Location/Qualifiers
     source          1..1015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(1..455)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(137..158)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     349..368
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        482..586
                     /gene="bla"
                     /label=AmpR promoter
     promoter        588..945
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        588..779
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(611..631)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      796..931
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
       61 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
      121 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
      181 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
      241 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
      301 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
      361 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
      421 acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt
      481 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      541 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag
      601 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
      661 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
      721 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
      781 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
      841 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
      901 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac
      961 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccg
//
