LOCUS       sequence-153182-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses lambdaN-HA-tagged human 4E-BP1 in Mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Peter D, Igreja C, Weber R, Wohlbold L, Weiler C, Ebertsch L, 
            Weichenrieder O, Izaurralde E
  TITLE     Molecular architecture of 4E-BP translational inhibitors bound to 
            eIF4E.
  JOURNAL   Mol Cell. 2015 Mar 19;57(6):1074-87. doi: 
            10.1016/j.molcel.2015.01.017. Epub 2015 Feb 19.
  PUBMED    25702871
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.01.017"; journalName: "Mol Cell"; date: 
            "2015-03-19- 19"; volume: "57"; issue: "6"; pages: "1074-87"
COMMENT     Sequence Label: pLNHA-C1-Hs4E-BP1
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             106..171
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             196..222
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    724..845
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(761..780)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     815..834
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(989..1010)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctctagcg ctaccggtcg ccaccatgga cgcacaaaca
      121 cgacgacgtg agcgtcgcgc tgagaaacaa gctcaatgga aagctgcaaa cggtggatct
      181 cctccacata tggcttaccc atacgatgtt ccagattacg ctcctccatc tcgagctatg
      241 tccgggggca gcagctgcag ccagacccca agccgggcca tccccgccac tcgccgggtg
      301 gtgctcggcg acggcgtgca gctcccgccc ggggactaca gcacgacccc cggcggcacg
      361 ctcttcagca ccaccccggg aggtaccagg atcatctatg accggaaatt cctgatggag
      421 tgtcggaact cacctgtgac caaaacaccc ccaagggatc tgcccaccat tccgggggtc
      481 accagccctt ccagtgatga gccccccatg gaagccagcc agagccacct gcgcaatagc
      541 ccagaagata agcgggcggg cggtgaagag tcacagtttg agatggacat ttaaggatcc
      601 accggatcta gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct
      661 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
      721 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
      781 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
      841 tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
      901 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
      961 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
//
