LOCUS       sequence-153203-         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the Ct biosensor under a T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Wang XC, Wilson SC, Hammond MC
  TITLE     Next-generation RNA-based fluorescent biosensors enable anaerobic 
            detection of cyclic di-GMP.
  JOURNAL   Nucleic Acids Res. 2016 Jul 5. pii: gkw580.
  PUBMED    27382070
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Jul 5. pii: gkw580."
COMMENT     Sequence Label: pET31b-T7-Spinach2-Ct
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     273..292
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        362..380
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      548..595
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             602..619
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(672..690)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      686..733
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(857..876)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga
       61 tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt
      121 agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg
      181 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg
      241 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
      301 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc
      361 ttaatacgac tcactatagg atgtaactga atgaaatggt gaaggacggg tccaatgaaa
      421 cagggcaaaa tcaccgaaag gtgatgacgc aaagccatgg gtctactgtt ttaaaacaat
      481 gttttaaagc tatgatcgcc aggctgccat ttgttgagta gagtgtgagc tccgtaacta
      541 gttacatcta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctcga
      601 gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt
      661 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
      721 gaggggtttt ttgctgaaag gaggaactat atccggattg gcgaatggga cgcgccctgt
      781 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
      841 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
      901 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
      961 cacctcgacc ccaaaaaact tgattagggt gatggtt
//
