LOCUS       sequence-153204-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the Ct biosensor under a T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Wang XC, Wilson SC, Hammond MC
  TITLE     Next-generation RNA-based fluorescent biosensors enable anaerobic 
            detection of cyclic di-GMP.
  JOURNAL   Nucleic Acids Res. 2016 Jul 5. pii: gkw580.
  PUBMED    27382070
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Jul 5. pii: gkw580."
COMMENT     Sequence Label: pET31b-T7-Spinach2-Ct
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(196..300)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(326..781)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(463..484)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     675..694
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(818..865)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     861..879
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(932..949)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      complement(956..1003)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
       61 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
      121 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
      181 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
      241 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
      301 cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg
      361 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
      421 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
      481 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
      541 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
      601 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
      661 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
      721 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
      781 tcccattcgc caatccggat atagttcctc ctttcagcaa aaaacccctc aagacccgtt
      841 tagaggcccc aaggggttat gctagttatt gctcagcggt ggcagcagcc aactcagctt
      901 cctttcgggc tttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagcaaaa
      961 aacccctcaa gacccgttta gaggccccaa ggggttatgc tagatgtaac ta
//
