LOCUS       sequence-153218-        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the Dp biosensor under a T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Wang XC, Wilson SC, Hammond MC
  TITLE     Next-generation RNA-based fluorescent biosensors enable anaerobic 
            detection of cyclic di-GMP.
  JOURNAL   Nucleic Acids Res. 2016 Jul 5. pii: gkw580.
  PUBMED    27382070
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Jul 5. pii: gkw580."
COMMENT     Sequence Label: pET31b-T7-Spinach2-Dp
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(194..298)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(324..779)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(461..482)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     673..692
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(816..863)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     859..877
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(930..947)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      complement(954..1000)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
       61 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
      121 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
      181 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
      241 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
      301 catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt
      361 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
      421 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
      481 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
      541 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
      601 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
      661 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
      721 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
      781 ccattcgcca atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta
      841 gaggccccaa ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc
      901 tttcgggctt tgttagcagc cggatctcaa tggtggtggt ggtggtgctc gagcaaaaaa
      961 cccctcaaga cccgtttaga ggccccaagg ggttatgcta tggcgcccga acagggactt
     1021 g
//
