LOCUS       sequence-153229-         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses RpBphP1-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Kaberniuk AA, Shemetov AA, Verkhusha VV
  TITLE     A bacterial phytochrome-based optogenetic system controllable with 
            near-infrared light.
  JOURNAL   Nat Methods. 2016 May 9. doi: 10.1038/nmeth.3864.
  PUBMED    27159085
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 9. doi: 10.1038/nmeth.3864."
COMMENT     Sequence Label: pKA-141
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     38..57
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        38..56
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        62..80
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     62..79
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 agagctggtt tagtgaaccg tcagatcact agaagcttaa tacgactcac tatagggtac
       61 gatttaggtg acactataga ataaggagcg atcgccatgg ctagcgtggc aggtcatgcc
      121 tctggcagcc ccgcattcgg gaccgccgat ctttcgaatt gcgaacgtga agagatccac
      181 ctcgccggct cgatccagcc gcatggcgcg cttctggtcg tcagcgagcc ggatcatcgc
      241 atcatccagg ccagcgccaa cgccgcggaa tttctgaatc tcggaagcgt gctcggcgtt
      301 ccgctcgccg agatcgacgg cgatctgttg atcaagatcc tgccgcatct cgatcccacc
      361 gccgaaggca tgccggtcgc ggtgcgctgc cggatcggca atccctccac ggagtacgac
      421 ggtctgatgc atcggcctcc ggaaggcggg ctgatcatcg agctcgaacg tgccggcccg
      481 ccgatcgatc tgtccggcac gctggcgccg gcgctggagc ggatccgcac ggcgggctcg
      541 ctgcgcgcgc tgtgcgatga caccgcgctg ctgtttcagc agtgcaccgg ctacgaccgg
      601 gtgatggtgt atcgcttcga cgagcagggc cacggcgaag tgttctccga gcgccacgtg
      661 cccgggctcg aatcctattt cggcaaccgc tatccgtcgt cggacattcc gcagatggcg
      721 cggcggctgt acgagcggca gcgcgtccgc gtgctggtcg acgtcagcta tcagccggtg
      781 ccgctggagc cgcggctgtc gccgctgacc gggcgcgatc tcgacatgtc gggctgcttc
      841 ctgcgctcga tgtcgcc
//
