LOCUS       sequence-153232-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses RpBphP1-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Kaberniuk AA, Shemetov AA, Verkhusha VV
  TITLE     A bacterial phytochrome-based optogenetic system controllable with 
            near-infrared light.
  JOURNAL   Nat Methods. 2016 May 9. doi: 10.1038/nmeth.3864.
  PUBMED    27159085
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 9. doi: 10.1038/nmeth.3864."
COMMENT     Sequence Label: pKA-141
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     22..40
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(119..829)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(397..416)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     666..684
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gcggccgccc caaggggtta tgctagttat tgctcagcgg tggcagcagc caactcagct
       61 tcctttcggg ctttgttagc agccggatca gcttgcatgc ctgcaggtcg actctagatt
      121 acttgtacag ctcgtccatg ccgccggtgg agtggcggcc ctcggcgcgt tcgtactgtt
      181 ccacgatggt gtagtcctcg ttgtgggagg tgatgtccaa cttgatgttg acgttgtagg
      241 cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt cttgacctca gcgtcgtagt
      301 ggccgccgtc cttcagcttc agcctctgct tgatctcgcc cttcagggcg ccgtcctcgg
      361 ggtacatccg ctcggaggag gcctcccagc ccatggtctt cttctgcatt acggggccgt
      421 cggaggggaa gttggtgccg cgcagcttca ccttgtagat gaactcgccg tcctgcaggg
      481 aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa gttcatcacg cgctcccact
      541 tgaagccctc ggggaaggac agcttcaagt agtcggggat gtcggcgggg tgcttcacgt
      601 aggccttgga gccgtacatg aactgagggg acaggatgtc ccaggcgaag ggcagggggc
      661 cacccttggt caccttcagc ttggcggtct gggtgccctc gtaggggcgg ccctcgccct
      721 cgccctcgat ctcgaactcg tggccgttca cggagccctc catgtgcacc ttgaagcgca
      781 tgaactcctt gatgatggcc atgttatcct cctcgccctt gctcaccata ct
//
