LOCUS       sequence-153234-         678 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for C-terminal fusion with 
            G3GFP (CaMV35S promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB451
FEATURES             Location/Qualifiers
     source          1..678
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(55..179)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(220..248)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
       61 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
      121 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt
      181 gataacagca tgagtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa
      241 ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca
      301 acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg
      361 ccaacacttg ttactacttt cgcttacggt gtgcagtgct tctcaagata cccagatcat
      421 atgaagcggc acgacttctt caagagcgcc atgcctgagg gatacgtgca ggagaggacc
      481 atcttcttca aggacgacgg gaactacaag acacgtgctg aagtcaagtt tgagggagac
      541 accctcgtca acaggatcga gcttaaggga atcgatttca aggaggacgg aaacatcctc
      601 ggccacaagt tggaatacaa cttcaactcc cacaacgtgt acatcatggc agacaaacaa
      661 aagaatggaa tcaaagtt
//
