LOCUS       sequence-153235-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PpsR2-mVenus-CAAX under CMVd1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Kaberniuk AA, Shemetov AA, Verkhusha VV
  TITLE     A bacterial phytochrome-based optogenetic system controllable with 
            near-infrared light.
  JOURNAL   Nat Methods. 2016 May 9. doi: 10.1038/nmeth.3864.
  PUBMED    27159085
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 9. doi: 10.1038/nmeth.3864."
COMMENT     Sequence Label: pKA-142
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     34..53
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        34..52
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        58..76
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     58..75
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ctggtttagt gaaccgtcag atcactagaa gcttaatacg actcactata gggtacgatt
       61 taggtgacac tatagaataa ggagcgatcg ccatggctag cgcgtcaaag tccgttcatg
      121 ccgacatcac ccttctgctc gatatggagg gtgtgattcg cgaagccacc ctgtctccga
      181 cgatggcggc cgagagcgtg gacggttggc tggggcgtcg ctggagcgac atcgccggcg
      241 ccgaaggcgg cgacaaggtt cgccgcatgg tcgaagacgc ccgccgcagc ggcatctcgg
      301 ctttccgcca gatcaatcag cctttcccga gcggcgtcga aatcccgatc gaattcacca
      361 cgatgctgct gggcgaccgc accggcatga tcgcggtcgg caagaacatg caggcggtca
      421 ccgagctgca ttcccggctg atcgctgcgc agcaggcgat ggagcgcgac tattggcggt
      481 tgcgtgaatt ggagactcgc taccgcctgg tgttcgacgc tgccgccgat gcggtgatga
      541 tcgtctccgc cggcgacatg cgcatcgtcg aagccaaccg ggcggcggtg aatgcgatca
      601 gccgcgtcga gcgcggcaat gacgaccttg cggggcgtga tttcctcgcc gaagtggcgg
      661 ctgccgatcg cgatgcggtg cgcgacatgc tggcccaggt gcgtcagcgc ggcaccgcac
      721 tcagcgtcct cgttcatctc ggccgttacg accgcgcctg gatgctgcgc ggttcgctga
      781 tgtcgtccga gcgtcgtcag gttt
//
