LOCUS       sequence-153241-         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses NLS-PpsR2-mVenus under CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Kaberniuk AA, Shemetov AA, Verkhusha VV
  TITLE     A bacterial phytochrome-based optogenetic system controllable with 
            near-infrared light.
  JOURNAL   Nat Methods. 2016 May 9. doi: 10.1038/nmeth.3864.
  PUBMED    27159085
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 9. doi: 10.1038/nmeth.3864."
COMMENT     Sequence Label: pCMV-160
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     142..160
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(242..493)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLERVTAAGITLGMDELYK"
     primer_bind     633..652
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
       61 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atctagagtc
      121 gcggccgccc caaggggtta tgctagttat tgctcagcgg tggcagcagc caactcagct
      181 tcctttcggg ctttgttagc agccggatca gcttgcatgc ctgcaggtcg actctagatt
      241 acttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgcgt tccagcagga
      301 ccatgtgatc gcgcttctcg ttggggtctt tgctcagctt ggactggtag cttaggtagt
      361 ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg
      421 cgagctgcac gccgccgtcc tcgatgttgt ggcggatctt gaagttggcc ttgatgccgt
      481 tcttctgctt gtcggcggtg atatagacgt tgtggctgtt gtagttgtac tccagcttgt
      541 gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca
      601 gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga
      661 agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct
      721 tcatgtggtc ggggtagcgg gcgaagcact gcaggccgta gcccagggtg gtcacgaggg
      781 tgggccaggg cacgggcagc ttgccggtgg tgcagatcag cttcagggtc agctt
//
