LOCUS       sequence-153244-         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses RpBphP1-mCherry-TetR under CMVd1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Kaberniuk AA, Shemetov AA, Verkhusha VV
  TITLE     A bacterial phytochrome-based optogenetic system controllable with 
            near-infrared light.
  JOURNAL   Nat Methods. 2016 May 9. doi: 10.1038/nmeth.3864.
  PUBMED    27159085
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 9. doi: 10.1038/nmeth.3864."
COMMENT     Sequence Label: pT2/SVNeo-103
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(124..831)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(399..418)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     668..686
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 tagcagctct aatgcgctgt taatcacttt acttttatct aatctagtca tggacccacc
       61 agcacttcta gaactaccgc cggcactacc gccagcactc ccgccggcgg acccaccagc
      121 actcttgtac agctcgtcca tgccgccggt ggagtggcgg ccctcggcgc gttcgtactg
      181 ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc aacttgatgt tgacgttgta
      241 ggcgccgggc agctgcacgg gcttcttggc cttgtaggtg gtcttgacct cagcgtcgta
      301 gtggccgccg tccttcagct tcagcctctg cttgatctcg cccttcaggg cgccgtcctc
      361 ggggtacatc cgctcggagg aggcctccca gcccatggtc ttcttctgca ttacggggcc
      421 gtcggagggg aagttggtgc cgcgcagctt caccttgtag atgaactcgc cgtcctgcag
      481 ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca cgcgctccca
      541 cttgaagccc tcggggaagg acagcttcaa gtagtcgggg atgtcggcgg ggtgcttcac
      601 gtaggccttg gagccgtaca tgaactgagg ggacaggatg tcccaggcga agggcagggg
      661 gccacccttg gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc
      721 ctcgccctcg atctcgaact cgtggccgtt cacggagccc tccatgtgca ccttgaagcg
      781 catgaactcc ttgatgatgg ccatgttatc ctcctcgccc ttgctcacca tactagtacc
      841 agcactacca gcactaccag cacta
//
