LOCUS       sequence-153248-         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses NLS-PpsR2-VP16 under CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Kaberniuk AA, Shemetov AA, Verkhusha VV
  TITLE     A bacterial phytochrome-based optogenetic system controllable with 
            near-infrared light.
  JOURNAL   Nat Methods. 2016 May 9. doi: 10.1038/nmeth.3864.
  PUBMED    27159085
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 9. doi: 10.1038/nmeth.3864."
COMMENT     Sequence Label: pCMV-104
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     142..160
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(242..475)
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     regulatory      625..634
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
       61 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atctagagtc
      121 gcggccgccc caaggggtta tgctagttat tgctcagcgg tggcagcagc caactcagct
      181 tcctttcggg ctttgttagc agccggatca gcttgcatgc ctgcaggtcg actctagatt
      241 acccaccgta ctcgtcaatt ccaagggcat cggtaaacat ctgctcaaac tcgaagtcgg
      301 ccatatccag agcgccgtag ggggcggagt cgtggggggt aaatcccgga cccggggaat
      361 ccccgtcccc caacatgtcc agatcgaaat cgtctagcgc gtcggcatgc gccatcgcca
      421 cgtcctcgcc gtctaagtgg agctcgtccc ccaggctgac atcggtcggg ggggccgtcg
      481 acagtctgcg cgtgtgtccc gcggggagaa aggacaggcg cggagccgcc agccccgcct
      541 cttcgggggc gtcgtcgtcc gggagatcga gcaggccctc gatggtagac ccgtaattgt
      601 ttttcgtacg cgcgcggctg tacgccatgg tggcgtccgg accaccagca ctaccaccag
      661 cactaccacc agcactacca ccagcactac caccagcact aagcttatcc tctgcgtcgt
      721 ctgaggcgcc ctctgcaccg tcggcagcag aagcaacgac acctttgtcg tcgaagccgt
      781 agctgtt
//
