LOCUS       sequence-153267-         712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gRNA to knock out expression of COSMC (C1GalT1C1) gene. The product 
            of this gene is a chaperone aiding in synthesis of Core1 structures 
            on O-linked glycans..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Stolfa G, Mondal N, Zhu Y, Yu X, Buffone A Jr, Neelamegham S
  TITLE     Using CRISPR-Cas9 to quantify the contributions of O-glycans, 
            N-glycans and Glycosphingolipids to human leukocyte-endothelium 
            adhesion.
  JOURNAL   Sci Rep. 2016 Jul 26;6:30392. doi: 10.1038/srep30392.
  PUBMED    27458028
REFERENCE   2  (bases 1 to 712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-07-26"; volume: "6:30392. doi"; pages: " 
            10.1038/srep30392"
COMMENT     Sequence Label: pX330-COSMC-KO
FEATURES             Location/Qualifiers
     source          1..712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..52
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             59..79
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cgacggagac tacaaggatc atgatattga ttacaaagac gatgacgata agatggcccc
       61 aaagaagaag cggaaggtcg gtatccacgg agtcccagca gccgacaaga agtacagcat
      121 cggcctggac atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt
      181 gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct
      241 gatcggagcc ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac
      301 cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag
      361 caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt
      421 ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc
      481 ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga
      541 caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca
      601 cttcctgatc gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca
      661 gctggtgcag acctacaacc agctgttcga ggaaaacccc atcaacgcca gc
//
