LOCUS       sequence-153270-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the Pl-A biosensor under a T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Wang XC, Wilson SC, Hammond MC
  TITLE     Next-generation RNA-based fluorescent biosensors enable anaerobic 
            detection of cyclic di-GMP.
  JOURNAL   Nucleic Acids Res. 2016 Jul 5. pii: gkw580.
  PUBMED    27382070
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Jul 5. pii: gkw580."
COMMENT     Sequence Label: pET31b-T7-Spinach2-Pl-A
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     272..291
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     375..394
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        375..393
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      634..680
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             687..704
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(757..775)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      771..818
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(942..961)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gaattgactc tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat
       61 ggtgtccggg atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta
      121 gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc
      181 ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga
      241 gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa
      301 ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct
      361 cgatcccgcg aaattaatac gactcactat aggggcccgg atagctcagt cggtagagca
      421 gcggccggat gtaactgaat gaaatggtga aggacgggtc cactcttcga taacggcaaa
      481 cttgtcgaaa gataaggacg caaagccaca gggccttctt gatgaaccgt caatggcagc
      541 ctggctaccg aagggttgtt gagtagagtg tgagctccgt aactagttac atccggccgc
      601 gggtccaggg ttcaagtccc tgttcgggcg ccatagcata accccttggg gcctctaaac
      661 gggtcttgag gggttttttg ctcgagcacc accaccacca ccactgagat ccggctgcta
      721 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
      781 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
      841 gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
      901 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
      961 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg gg
//
