LOCUS       sequence-153271-        6933 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  overexpression of ID2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6933)
  AUTHORS   Del Real MM, Rothenberg EV
  TITLE     Architecture of a lymphomyeloid developmental switch controlled by 
            PU.1, Notch and Gata3.
  JOURNAL   Development. 2013 Mar;140(6):1207-19. doi: 10.1242/dev.088559.
  PUBMED    23444353
REFERENCE   2  (bases 1 to 6933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2013-03"; volume: "140"; issue: 
            "6"; pages: "1207-19"
COMMENT     Sequence Label: Id2 Ngfr
FEATURES             Location/Qualifiers
     source          1..6933
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2..21)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(681..1472,1473..1541))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1304..1323
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1542..1646)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1714..1732
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1770..1792)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1892..1911
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2105..2127
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2255..2274
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             2474..2990
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    3054..3395
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             3462..3878
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3808..3830
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3846..3862
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    4318..4885
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4335..4887
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4479..4496)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4706..4725
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     LTR             5801..6315
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6477..6493
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6501..6507,6508..6525,6526..6531))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6546..6567
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6684..6701)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(6855..6933,1..510))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
       61 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      121 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      181 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      241 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      301 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      361 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
      421 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
      481 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
      541 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
      601 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
      661 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
      721 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
      781 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
      841 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
      901 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
      961 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     1021 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     1081 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     1141 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     1201 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     1261 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     1321 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     1381 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     1441 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     1501 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     1561 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     1621 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     1681 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     1741 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     1801 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     1861 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
     1921 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
     1981 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     2041 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     2101 ttttcccagt cacgacgttg taaaacgacg gcgcaaggaa tggtgcatgc aaggagatgg
     2161 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
     2221 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
     2281 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggcgattag
     2341 tccaatttgt taaagacagg atatcagtgg tccaggctct agttttgact caacaatatc
     2401 accagctgaa gcctatagag tacgagccat agataaaata aaagatttta tttagtctcc
     2461 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc
     2521 cattttgcaa ggcatggaaa atacataact gagaatagag aagttcagat caaggttagg
     2581 aacagagaga cagcagaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
     2641 ggctcagggc caagaacaga tggtccccag atgcggtccc gccctcagca gtttctagag
     2701 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa
     2761 ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa
     2821 aagagcccac aacccctcac tcggcgcgcc agtcctccga tagactgcgt cgcccgggta
     2881 cccgtattcc caataaagcc tcttgctgtt tgcatccgaa tcgtggactc gctgatcctt
     2941 gggagggtct cctcagattg attgactgcc cacctcgggg gtctttcatt tggaggttcc
     3001 accgagattt ggagacccct gcccagggac caccgacccc cccgccggga ggtaagctgg
     3061 ccagcggtcg tttcgtgtct gtctctgtct ttgtgcgtgt ttgtgccggc atctaatgtt
     3121 tgcgcctgcg tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
     3181 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
     3241 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
     3301 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
     3361 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
     3421 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
     3481 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     3541 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     3601 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     3661 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     3721 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     3781 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     3841 cctcccttta tccagccctc actccttctc taggcgccgg aattagatct atgaaagcct
     3901 tcagtccggt gaggtccgtt aggaaaaaca gcctgtcgga ccacagcttg ggcatctccc
     3961 ggagcaaaac cccggtggac gacccgatga gtctgctcta caacatgaac gactgctact
     4021 ccaagctcaa ggaactggtg cccagcatcc cccagaacaa gaaggtgacc aagatggaaa
     4081 tcctgcagca cgtcatcgat tacatcttgg acctgcagat cgccctggac tcgcatccca
     4141 ctatcgtcag cctgcatcac cagagacctg gacagaacca ggcgtccagg acgccgctga
     4201 ccaccctgaa cacggacatc agcatcctgt ccttgcaggc atctgaattc ccttctgagc
     4261 ttatgtcgaa tgatagcaaa gtactctgtg gctaaagatc tctcgaggtt aacgaattcc
     4321 gccccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     4381 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     4441 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     4501 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     4561 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     4621 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     4681 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     4741 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
     4801 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     4861 tttcctttga aaaacacgat gataatatgg ccacaaccat ggacgggccg cgcctgctgc
     4921 tgttgctgct tctgggggtg tcccttggag gtgccaagga ggcatgcccc acaggcctgt
     4981 acacacacag cggtgagtgc tgcaaagcct gcaacctggg cgagggtgtg gcccagcctt
     5041 gtggagccaa ccagaccgtg tgtgagccct gcctggacag cgtgacgttc tccgacgtgg
     5101 tgagcgcgac cgagccgtgc aagccgtgca ccgagtgcgt ggggctccag agcatgtcgg
     5161 cgccgtgcgt ggaggccgac gacgccgtgt gccgctgcgc ctacggctac taccaggatg
     5221 agacgactgg gcgctgcgag gcgtgccgcg tgtgcgaggc gggctcgggc ctcgtgttct
     5281 cctgccagga caagcagaac accgtgtgcg aggagtgccc cgacggcacg tattccgacg
     5341 aggccaacca cgtggacccg tgcctgccct gcaccgtgtg cgaggacacc gagcgccagc
     5401 tccgcgagtg cacacgctgg gccgacgccg agtgcgagga gatccctggc cgttggatta
     5461 cacggtccac acccccagag ggctcggaca gcacagcccc cagcacccag gagcctgagg
     5521 cacctccaga acaagacctc atagccagca cggtggcagg tgtggtgacc acagtgatgg
     5581 gcagctccca gcccgtggtg acccgaggca ccaccgacaa cctcatccct gtctattgct
     5641 ccatcctggc tgctgtggtt gtgggccttg tggcctacat agccttcaag aggtggaaca
     5701 gtcatcgata tcctcgaggt caccgcggtc tagagtcgac ctgcagccaa gcttatcgat
     5761 aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag
     5821 gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaatac ataactgaga
     5881 atagagaagt tcagatcaag gttaggaaca gagagacagc agaatatggg ccaaacagga
     5941 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc
     6001 ggtcccgccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc
     6061 tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc
     6121 gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg cgcgccagtc
     6181 ctccgataga ctgcgtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat
     6241 ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca
     6301 gcgggggtct ttcatgggta acagtttctt gaagttggag aacaacattc tgagggtagg
     6361 agtcgaatat taagtaatcc tgactcaatt agccactgtt ttgaatccac atactccaat
     6421 actcctgaaa tagttcatta tggacagcgc agaaagagct ggggagaatt gtgaaattgt
     6481 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     6541 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     6601 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     6661 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     6721 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     6781 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     6841 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     6901 tcaagtcaga ggtggcgaaa cccgacagga cta
//
