LOCUS       sequence-153276-         516 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gRNA to knock out expression of UGCG gene. The product of this gene 
            catalyzes the first step in synthesis of glycosphingolipids..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Stolfa G, Mondal N, Zhu Y, Yu X, Buffone A Jr, Neelamegham S
  TITLE     Using CRISPR-Cas9 to quantify the contributions of O-glycans, 
            N-glycans and Glycosphingolipids to human leukocyte-endothelium 
            adhesion.
  JOURNAL   Sci Rep. 2016 Jul 26;6:30392. doi: 10.1038/srep30392.
  PUBMED    27458028
REFERENCE   2  (bases 1 to 516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-07-26"; volume: "6:30392. doi"; pages: " 
            10.1038/srep30392"
COMMENT     Sequence Label: pX330-UGCG-KO
FEATURES             Location/Qualifiers
     source          1..516
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        25..100
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        197..482
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 caccgctgtg gctgatgcat ttcagtttta gagctagaaa tagcaagtta aaataaggct
       61 agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttttgttt tagagctaga
      121 aatagcaagt taaaataagg ctagtccgtt tttagcgcgt gcgccaattc tgcagacaaa
      181 tggctctaga ggtacccgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      241 acgacccccg cccattgacg tcaatagtaa cgccaatagg gactttccat tgacgtcaat
      301 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      361 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattgt gcccagtaca
      421 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      481 tggtcgaggt gagccccacg ttctgcttca ctctcc
//
