LOCUS       sequence-153284-        7352 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  overexpression of Pu.1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7352)
  AUTHORS   Del Real MM, Rothenberg EV
  TITLE     Architecture of a lymphomyeloid developmental switch controlled by 
            PU.1, Notch and Gata3.
  JOURNAL   Development. 2013 Mar;140(6):1207-19. doi: 10.1242/dev.088559.
  PUBMED    23444353
REFERENCE   2  (bases 1 to 7352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2013-03"; volume: "140"; issue: 
            "6"; pages: "1207-19"
COMMENT     Sequence Label: Pu.1 NGFR
FEATURES             Location/Qualifiers
     source          1..7352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2..21)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(681..1472,1473..1541))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1304..1323
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1542..1646)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1714..1732
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1770..1792)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1892..1911
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2105..2127
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2255..2274
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             2474..2990
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    3054..3395
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             3462..3878
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3808..3830
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3846..3862
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    4737..5304
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4754..5306
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4898..4915)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5125..5144
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     LTR             6220..6734
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6896..6912
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6920..6926,6927..6944,6945..6950))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6965..6986
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7103..7120)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(7274..7352,1..510))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
       61 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      121 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      181 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      241 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      301 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      361 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
      421 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
      481 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
      541 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
      601 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
      661 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
      721 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
      781 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
      841 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
      901 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
      961 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     1021 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     1081 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     1141 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     1201 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     1261 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     1321 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     1381 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     1441 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     1501 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     1561 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     1621 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     1681 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     1741 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     1801 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     1861 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
     1921 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
     1981 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     2041 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     2101 ttttcccagt cacgacgttg taaaacgacg gcgcaaggaa tggtgcatgc aaggagatgg
     2161 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
     2221 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
     2281 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggcgattag
     2341 tccaatttgt taaagacagg atatcagtgg tccaggctct agttttgact caacaatatc
     2401 accagctgaa gcctatagag tacgagccat agataaaata aaagatttta tttagtctcc
     2461 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc
     2521 cattttgcaa ggcatggaaa atacataact gagaatagag aagttcagat caaggttagg
     2581 aacagagaga cagcagaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
     2641 ggctcagggc caagaacaga tggtccccag atgcggtccc gccctcagca gtttctagag
     2701 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa
     2761 ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa
     2821 aagagcccac aacccctcac tcggcgcgcc agtcctccga tagactgcgt cgcccgggta
     2881 cccgtattcc caataaagcc tcttgctgtt tgcatccgaa tcgtggactc gctgatcctt
     2941 gggagggtct cctcagattg attgactgcc cacctcgggg gtctttcatt tggaggttcc
     3001 accgagattt ggagacccct gcccagggac caccgacccc cccgccggga ggtaagctgg
     3061 ccagcggtcg tttcgtgtct gtctctgtct ttgtgcgtgt ttgtgccggc atctaatgtt
     3121 tgcgcctgcg tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
     3181 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
     3241 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
     3301 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
     3361 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
     3421 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
     3481 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     3541 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     3601 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     3661 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     3721 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     3781 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     3841 cctcccttta tccagccctc actccttctc taggcgccgg aattagatct cacatgttac
     3901 aggcgtgcaa aatggaaggg ttttccctca ccgcccctcc atcggatgac ttggttactt
     3961 acgattcaga gctataccaa cgtccaatgc atgactacta ctccttcgtg ggcagcgatg
     4021 gagaaagcca tagcgatcac tactgggatt tctccgcaca ccatgtccac aacaacgagt
     4081 ttgagaactt ccctgagaac cacttcacag agctgcagag cgtgcagccc ccgcagctac
     4141 agcagctcta tcgccacatg gagctggaac agatgcacgt cctcgatact cccatggtgc
     4201 caccccacac cggcctcagt caccaggttt cctacatgcc ccggatgtgc ttcccttatc
     4261 aaaccttgtc cccagcccac cagcagagct cagatgagga ggagggtgag aggcagagcc
     4321 ctcccctgga ggtgtctgat ggagaagctg atggcttgga gcctgggcca ggtcttctgc
     4381 acggggagac aggcagcaag aaaaagattc gcctgtacca gttcctgctg gacctgctgc
     4441 gcagcggcga catgaaggac agcatctggt gggtggacaa ggacaaaggt accttccagt
     4501 tctcgtccaa gcacaaggag gcgctggcgc accgctgggg catccagaag ggcaaccgca
     4561 agaagatgac ctaccagaag atggcgcgcg cgctgcgcaa ctacggcaag acaggcgagg
     4621 tgaagaaagt caagaagaag ctcacctacc agttcagcgg cgaggtgctg ggccgtgggg
     4681 gcctggccga gcggcgcctc ccgccccact gatcgcggcc gctacgtaaa ttgaattccg
     4741 cccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
     4801 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     4861 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     4921 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     4981 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     5041 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     5101 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
     5161 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
     5221 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
     5281 ttcctttgaa aaacacgatg ataatatggc cacaaccatg gacgggccgc gcctgctgct
     5341 gttgctgctt ctgggggtgt cccttggagg tgccaaggag gcatgcccca caggcctgta
     5401 cacacacagc ggtgagtgct gcaaagcctg caacctgggc gagggtgtgg cccagccttg
     5461 tggagccaac cagaccgtgt gtgagccctg cctggacagc gtgacgttct ccgacgtggt
     5521 gagcgcgacc gagccgtgca agccgtgcac cgagtgcgtg gggctccaga gcatgtcggc
     5581 gccgtgcgtg gaggccgacg acgccgtgtg ccgctgcgcc tacggctact accaggatga
     5641 gacgactggg cgctgcgagg cgtgccgcgt gtgcgaggcg ggctcgggcc tcgtgttctc
     5701 ctgccaggac aagcagaaca ccgtgtgcga ggagtgcccc gacggcacgt attccgacga
     5761 ggccaaccac gtggacccgt gcctgccctg caccgtgtgc gaggacaccg agcgccagct
     5821 ccgcgagtgc acacgctggg ccgacgccga gtgcgaggag atccctggcc gttggattac
     5881 acggtccaca cccccagagg gctcggacag cacagccccc agcacccagg agcctgaggc
     5941 acctccagaa caagacctca tagccagcac ggtggcaggt gtggtgacca cagtgatggg
     6001 cagctcccag cccgtggtga cccgaggcac caccgacaac ctcatccctg tctattgctc
     6061 catcctggct gctgtggttg tgggccttgt ggcctacata gccttcaaga ggtggaacag
     6121 tcatcgatat cctcgaggtc accgcggtct agagtcgacc tgcagccaag cttatcgata
     6181 aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg
     6241 tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaataca taactgagaa
     6301 tagagaagtt cagatcaagg ttaggaacag agagacagca gaatatgggc caaacaggat
     6361 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
     6421 gtcccgccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
     6481 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
     6541 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
     6601 tccgatagac tgcgtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
     6661 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
     6721 cgggggtctt tcatgggtaa cagtttcttg aagttggaga acaacattct gagggtagga
     6781 gtcgaatatt aagtaatcct gactcaatta gccactgttt tgaatccaca tactccaata
     6841 ctcctgaaat agttcattat ggacagcgca gaaagagctg gggagaattg tgaaattgtt
     6901 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     6961 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7021 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7081 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7141 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7201 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7261 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7321 caagtcagag gtggcgaaac ccgacaggac ta
//
