LOCUS       sequence-153285-         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Monomeric near-infrared fluorescent protein miRFP709 in mammalian 
            plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Shcherbakova DM, Baloban M, Emelyanov AV, Brenowitz M, Guo P, 
            Verkhusha VV
  TITLE     Bright monomeric near-infrared fluorescent proteins as tags and 
            biosensors for multiscale imaging.
  JOURNAL   Nat Commun. 2016 Aug 19;7:12405. doi: 10.1038/ncomms12405.
  PUBMED    27539380
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-08-19"; volume: "7:12405. doi"; pages: " 
            10.1038/ncomms12405"
COMMENT     Sequence Label: pmiRFP709-N1
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    12..92
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      94..103
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ccgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttcg aattctgcag
       61 tcgacggtac cgcgggcccg ggatccaccg gtcgccacca tggtagcagg tcatgcctct
      121 ggcagccccg cattcgggac cgcctctcat tcgaattgcg aacatgaaga gatccacctc
      181 gccggctcga tccagccgca tggcgcgctt ctggtcgtca gcgaacatga tcatcgcgtc
      241 atccaggcca gcgccaacgc cgcggaattt ctgaatctcg gaagcgtact cggcgttccg
      301 ctcgccgaga tcgacggcga tctgttgatc aagatcctgc cgcatctcga tcccaccgcc
      361 gaaggcatgc cggtcgcggt gcgctgccgg atcggcaatc cctctacgga gtactgcggt
      421 ctgatgcatc ggcctccgga aggcgggctg atcatcgaac tcgaacgtgc cggcccgtcg
      481 atcgatctgt caggcacgct ggcgccggcg ctggagcgga tccgcacggc gggttcactg
      541 cgcgcgctgt gcgatgacac cgtgctgctg tttcagcagt gcaccggcta cgaccgggtg
      601 atggtgtatc gtttcgatga gcaaggccac ggcctggtat tctccgagtg ccatgtgcct
      661 gggctcgaat cctatttcgg caaccgctat ccgtcgtcgt tcatcccgca gatggcgcgg
      721 cagctgtacg tgcggcagcg cgtccgcgtg ctggtcgacg tcacctatca gccggtgccg
      781 ctggagccgc ggctgtcgcc gctgaccggg cgcgatctcg acatgtcgg
//
