LOCUS       sequence-153287-         452 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gRNA to knock out expression of MGAT1 gene. The product of this gene
            is essential for synthesis of complex and hybrid N-Glycans..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Stolfa G, Mondal N, Zhu Y, Yu X, Buffone A Jr, Neelamegham S
  TITLE     Using CRISPR-Cas9 to quantify the contributions of O-glycans, 
            N-glycans and Glycosphingolipids to human leukocyte-endothelium 
            adhesion.
  JOURNAL   Sci Rep. 2016 Jul 26;6:30392. doi: 10.1038/srep30392.
  PUBMED    27458028
REFERENCE   2  (bases 1 to 452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-07-26"; volume: "6:30392. doi"; pages: " 
            10.1038/srep30392"
COMMENT     Sequence Label: pX330-MGAT1-KO
FEATURES             Location/Qualifiers
     source          1..452
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        19..94
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ggggcgctat cctctttggt tttagagcta gaaatagcaa gttaaaataa ggctagtccg
       61 ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt gttttagagc tagaaatagc
      121 aagttaaaat aaggctagtc cgtttttagc gcgtgcgcca attctgcaga caaatggctc
      181 tagaggtacc cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      241 cccgcccatt gacgtcaata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttgtgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct ac
//
