LOCUS       sequence-153293-         610 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gRNA to knock out expression of MGAT1 gene. The product of this gene
            is essential for synthesis of complex and hybrid N-Glycans..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Stolfa G, Mondal N, Zhu Y, Yu X, Buffone A Jr, Neelamegham S
  TITLE     Using CRISPR-Cas9 to quantify the contributions of O-glycans, 
            N-glycans and Glycosphingolipids to human leukocyte-endothelium 
            adhesion.
  JOURNAL   Sci Rep. 2016 Jul 26;6:30392. doi: 10.1038/srep30392.
  PUBMED    27458028
REFERENCE   2  (bases 1 to 610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-07-26"; volume: "6:30392. doi"; pages: " 
            10.1038/srep30392"
COMMENT     Sequence Label: pX330-MGAT1-KO
FEATURES             Location/Qualifiers
     source          1..610
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..38
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atgacgataa gatggcccca aagaagaagc ggaaggtcgg tatccacgga gtcccagcag
       61 ccgacaagaa gtacagcatc ggcctggaca tcggcaccaa ctctgtgggc tgggccgtga
      121 tcaccgacga gtacaaggtg cccagcaaga aattcaaggt gctgggcaac accgaccggc
      181 acagcatcaa gaagaacctg atcggagccc tgctgttcga cagcggcgaa acagccgagg
      241 ccacccggct gaagagaacc gccagaagaa gatacaccag acggaagaac cggatctgct
      301 atctgcaaga gatcttcagc aacgagatgg ccaaggtgga cgacagcttc ttccacagac
      361 tggaagagtc cttcctggtg gaagaggata agaagcacga gcggcacccc atcttcggca
      421 acatcgtgga cgaggtggcc taccacgaga agtaccccac catctaccac ctgagaaaga
      481 aactggtgga cagcaccgac aaggccgacc tgcggctgat ctatctggcc ctggcccaca
      541 tgatcaagtt ccggggccac ttcctgatcg agggcgacct gaaccccgac aacagcgacg
      601 tggacaagct
//
