LOCUS       sequence-153294-        1037 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral expression of random shRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1037)
  TITLE     Rothenberg lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Banshee shRandom mCherry
FEATURES             Location/Qualifiers
     source          1..1037
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..23
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      85..94
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             91..801
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(236..254)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     504..523
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     907..926
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 tcctcccttt atccagccct cactccttct ctaggcgccg gaattctgca gtcgacggta
       61 ccgcgggccc gggatccacc ggtcgccacc atggtgagca agggcgagga ggataacatg
      121 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
      181 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
      241 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
      301 atgtacggct ccaaggccta cgtgaagcac cccgccgaca ttcccgacta cttgaagctg
      361 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
      421 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
      481 ggcaccaact tcccctccga cggccccgta atgcagaaga agacaatggg ctgggaggcc
      541 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg
      601 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag
      661 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac
      721 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc
      781 atggacgagc tgtacaagta agccatggcg atgcctgctt gccgaatatc atggtggaaa
      841 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
      901 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
      961 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     1021 ttgacgagtt cttctga
//
