LOCUS       sequence-153302-        1097 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses shRNA targeting TCF1 and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1097)
  TITLE     Rothenberg lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Banshee shTCF1 mCherry
FEATURES             Location/Qualifiers
     source          1..1097
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..22
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      84..93
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             90..800
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(235..253)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     503..522
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     906..925
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 cctcccttta tccagccctc actccttctc taggcgccgg aattctgcag tcgacggtac
       61 cgcgggcccg ggatccaccg gtcgccacca tggtgagcaa gggcgaggag gataacatgg
      121 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
      181 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
      241 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
      301 tgtacggctc caaggcctac gtgaagcacc ccgccgacat tcccgactac ttgaagctgt
      361 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
      421 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
      481 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gacaatgggc tgggaggcct
      541 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
      601 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
      661 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
      721 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
      781 tggacgagct gtacaagtaa gccatggcga tgcctgcttg ccgaatatca tggtggaaaa
      841 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
      901 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
      961 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     1021 tgacgagttc ttctgagcgg gactctgggg ttcggtagtc gacggatcct ctagacgcgt
     1081 ctcgagtcga cggtatc
//
