LOCUS       sequence-153303-         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  F-Actin labeling with near-infrared fluorescent protein miRFP703.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Shcherbakova DM, Baloban M, Emelyanov AV, Brenowitz M, Guo P, 
            Verkhusha VV
  TITLE     Bright monomeric near-infrared fluorescent proteins as tags and 
            biosensors for multiscale imaging.
  JOURNAL   Nat Commun. 2016 Aug 19;7:12405. doi: 10.1038/ncomms12405.
  PUBMED    27539380
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-08-19"; volume: "7:12405. doi"; pages: " 
            10.1038/ncomms12405"
COMMENT     Sequence Label: pLifeAct-miRFP703
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             37..87
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      103..112
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctggtttagt gaaccgtcag atccgctagc gccaccatgg gcgtggccga cttgatcaag
       61 aagttcgagt ccatctccaa ggaggagggg gatccaccgg tcgccaccat ggtagcaggt
      121 catgcctctg gcagccccgc attcgggacc gcctctcatt cgaattgcga acatgaagag
      181 atccacctcg ccggctcgat ccagccgcat ggcgcgcttc tggtcgtcag cgaacatgat
      241 catcgcgtca tccaggccag cgccaacgcc gcggaatttc tgaatctcgg aagcgtactc
      301 ggcgttccgc tcgccgagat cgacggcgat ctgttgatca agatcctgcc gcatctcgat
      361 cccaccgccg aaggcatgcc ggtcgcggtg cgctgccgga tcggcaatcc ctctacggag
      421 tactgcggtc tgatgcatcg gcctccggaa ggcgggctga tcatcgaact cgaacgtgcc
      481 ggcccgtcga tcgatctgtc aggcacgctg gcgccggcgc tggagcggat ccgcacggcg
      541 ggttcactgc gcgcgctgtg cgatgacacc gtgctgctgt ttcagcagtg caccggctac
      601 gaccgggtga tggtgtatcg tttcgatgag caaggccacg gcctggtatt ctccgagtgc
      661 catgtgcctg ggctcgaatc ctatttcggc aaccgctatc cgtcgtcgct ggtcccgcag
      721 atggcgcggc agctgtacgt gcggcagcgc gtccgcgtgc tggtcgacgt cacctatcag
      781 ccggtgccgc tggagccgcg gctgtcgccg ctgaccgggc gcgatctcga catgtcgggc
      841 tgcttcctgc gctcgatgtc gccgatccat ctgcagttcc tgaaggacat gggcgtgcgc
      901 gc
//
