LOCUS       sequence-153319-         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the Pl-B biosensor under a T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Wang XC, Wilson SC, Hammond MC
  TITLE     Next-generation RNA-based fluorescent biosensors enable anaerobic 
            detection of cyclic di-GMP.
  JOURNAL   Nucleic Acids Res. 2016 Jul 5. pii: gkw580.
  PUBMED    27382070
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Jul 5. pii: gkw580."
COMMENT     Sequence Label: pET31b-T7-Spinach2-Pl-B
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     273..292
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     376..395
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        376..394
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      631..677
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             684..701
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(754..772)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      768..815
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(939..958)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga
       61 tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt
      121 agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg
      181 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg
      241 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
      301 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc
      361 tcgatcccgc gaaattaata cgactcacta taggggcccg gatagctcag tcggtagagc
      421 agcggccgga tgtaactgaa tgaaatggtg aaggacgggt ccacttcgat aacggcaaac
      481 ttgtcgaaag ataaggacgc aaagccacag ggccttcttg atgaaccgtc aatggcagcc
      541 tggctaccga agttgttgag tagagtgtga gctccgtaac tagttacatc cggccgcggg
      601 tccagggttc aagtccctgt tcgggcgcca tagcataacc ccttggggcc tctaaacggg
      661 tcttgagggg ttttttgctc gagcaccacc accaccacca ctgagatccg gctgctaaca
      721 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc
      781 ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
      841 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
      901 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      961 ctttctcgcc acgttcgccg g
//
