LOCUS       sequence-153321-         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the Pl-B biosensor under a T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Wang XC, Wilson SC, Hammond MC
  TITLE     Next-generation RNA-based fluorescent biosensors enable anaerobic 
            detection of cyclic di-GMP.
  JOURNAL   Nucleic Acids Res. 2016 Jul 5. pii: gkw580.
  PUBMED    27382070
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Jul 5. pii: gkw580."
COMMENT     Sequence Label: pET31b-T7-Spinach2-Pl-B
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(171..275)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(301..756)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(438..459)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     650..669
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(793..840)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     836..854
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(907..924)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
       61 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
      121 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
      181 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
      241 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
      301 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
      361 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      421 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      481 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      541 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      601 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      661 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      721 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccaatc cggatatagt
      781 tcctcctttc agcaaaaaac ccctcaagac ccgtttagag gccccaaggg gttatgctag
      841 ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt tagcagccgg
      901 atctcagtgg tggtggtggt ggtgctcgag caaaaaaccc ctcaagaccc gtttagaggc
      961 cccaaggggt tatgc
//
