LOCUS       sequence-153333-         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Histone 2B labeling with near-infrared fluorescent protein miRFP703.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Shcherbakova DM, Baloban M, Emelyanov AV, Brenowitz M, Guo P, 
            Verkhusha VV
  TITLE     Bright monomeric near-infrared fluorescent proteins as tags and 
            biosensors for multiscale imaging.
  JOURNAL   Nat Commun. 2016 Aug 19;7:12405. doi: 10.1038/ncomms12405.
  PUBMED    27539380
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-08-19"; volume: "7:12405. doi"; pages: " 
            10.1038/ncomms12405"
COMMENT     Sequence Label: pH2B-miRFP703
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             90..467
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      480..489
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tggtttagtg aaccgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt
       61 cgaattctgc agtcgacggt accgccacca tgccagagcc agcgaagtct gctcccgccc
      121 cgaaaaaggg ctccaagaag gcggtgacta aggcgcagaa gaaaggcggc aagaagcgca
      181 agcgcagccg caaggagagc tattccatct atgtgtacaa ggttctgaag caggtccacc
      241 ctgacaccgg catttcgtcc aaggccatgg gcatcatgaa ttcgtttgtg aacgacattt
      301 tcgagcgcat cgcaggtgag gcttcccgcc tggcgcatta caacaagcgc tcgaccatca
      361 cctccaggga gatccagacg gccgtgcgcc tgctgctgcc tggggagttg gccaagcacg
      421 ccgtgtccga gggtactaag gccatcacca agtacaccag cgctaaggat ccaccggtcg
      481 ccaccatggt agcaggtcat gcctctggca gccccgcatt cgggaccgcc tctcattcga
      541 attgcgaaca tgaagagatc cacctcgccg gctcgatcca gccgcatggc gcgcttctgg
      601 tcgtcagcga acatgatcat cgcgtcatcc aggccagcgc caacgccgcg gaatttctga
      661 atctcggaag cgtactcggc gttccgctcg ccgagatcga cggcgatctg ttgatcaaga
      721 tcctgccgca tctcgatccc accgccgaag gcatgccggt cgcggtgcgc tgccggatcg
      781 gcaatccctc tacggagtac tgcggtctga tgcatcg
//
