LOCUS       sequence-153347-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP2
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             48..71
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             84..110
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cagccctcac tccttctcta ggcgccggaa ttagatctgc caccatggac tacaaggatg
       61 acgatgacaa gctcgatgga ggatacccat acgatgttcc agattacgct gctcgaggtt
      121 atcaaacaag tttgtacaaa aaagttggca tgtcccagct ctcctccacc ctgaagcgct
      181 acacagaatc ggcccgctac acagatgccc actatgccaa gtcgggctat ggtgcctaca
      241 ccccatcctc ctatggggcc aatctggctg cctccttact ggagaaggag aaacttggtt
      301 tcaagccggt ccccaccagc agcttcctca cccgtccccg tacctatggc ccctcctccc
      361 tcctggacta tgaccggggc cgccccctgc tgagacccga catcactggg ggtggtaagc
      421 gggcagagag ccagacccgg ggtactgagc ggcctttagg cagtggcctc agcgggggca
      481 gcggattccc ttatggagtg accaacaact gcctcagcta cctgcccatc aatgcctatg
      541 accagggggt gaccctaacc cagaagctgg acagccaatc agacctggcc cgggatttct
      601 ccagcctccg gacctcagat agctaccgga tagaccccag gaacctgggc cgcagcccca
      661 tgctggcccg gacgcgcaag gagctctgca ccctgcaggg gctctaccag acagccagct
      721 gccctgaata cctggtcgac tacctggaga actatggtcg caagggcagt gcatctcagg
      781 tgccctccca ggcccctccc tcacgagtcc ctgaaatcat cagcccaacc taccgaccca
      841 ttggccgcta cacgctgtgg gagacggg
//
