LOCUS       sequence-153348-         317 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human NEUROGENIN2 (hNGN2), eGFP and puromycin resistance 
            gene under control of TetON promoter. This 3rd generation lentiviral
            vector is used to generate NGN2-iNs from hiPSCs and hiPSC-NPCs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Ho SM, Hartley BJ, Tcw J, Beaumont M, Stafford K, Slesinger PA, 
            Brennand KJ
  TITLE     Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural
            progenitor cells.
  JOURNAL   Methods. 2015 Nov 25. pii: S1046-2023(15)30159-6. doi: 
            10.1016/j.ymeth.2015.11.019.
  PUBMED    26626326
REFERENCE   2  (bases 1 to 317)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            2015 Nov 25. pii: S1046-2023(15)30159-6. doi: 
            10.1016/j.ymeth.2015.11.019."
COMMENT     Sequence Label: pLV-TetO-hNGN2-eGFP-Puro
FEATURES             Location/Qualifiers
     source          1..317
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agacaccggg accgatccag cctccgcggc cccgaattcg ccaccatgtt cgtcaaatcc
       61 gagaccttgg agttgaagga ggaagaggac gtgttagtgc tgctcggatc ggcctccccc
      121 gccttggcgg ccctgacccc gctgtcatcc agcgccgacg aagaagagga ggaggagccg
      181 ggcgcgtcag gcggggcgcg tcggcagcgc ggggctgagg ccgggcaggg ggcgcggggc
      241 ggcgtggctg cgggtgcgga gggctgccgg cccgcacggc tgctgggtct ggtacacgat
      301 tgcaaacggc gcccttc
//
