LOCUS       sequence-153349-         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human NEUROGENIN2 (hNGN2), eGFP and puromycin resistance 
            gene under control of TetON promoter. This 3rd generation lentiviral
            vector is used to generate NGN2-iNs from hiPSCs and hiPSC-NPCs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Ho SM, Hartley BJ, Tcw J, Beaumont M, Stafford K, Slesinger PA, 
            Brennand KJ
  TITLE     Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural
            progenitor cells.
  JOURNAL   Methods. 2015 Nov 25. pii: S1046-2023(15)30159-6. doi: 
            10.1016/j.ymeth.2015.11.019.
  PUBMED    26626326
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            2015 Nov 25. pii: S1046-2023(15)30159-6. doi: 
            10.1016/j.ymeth.2015.11.019."
COMMENT     Sequence Label: pLV-TetO-hNGN2-eGFP-Puro
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..74
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             87..680
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="EYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVT
                     ELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQ
                     QQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAP
                     RNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     577..597
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(772..792)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 agctgtacaa gggttcaggg gagggaagag gttccctgct cacatgcgga gacgtagaag
       61 agaatcccgg acccatgacc tctagagagt acaagcccac ggtgcgcctc gccacccgcg
      121 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
      181 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
      241 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
      301 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
      361 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
      421 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
      481 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
      541 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
      601 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
      661 cccgcaagcc cggtgcctga ttaattaagc tagctaattc gatatcaagc ttatcgataa
      721 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
      781 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
      841 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg a
//
