LOCUS       sequence-153351-         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP14
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             48..71
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             84..110
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cagccctcac tccttctcta ggcgccggaa ttagatctgc caccatggac tacaaggatg
       61 acgatgacaa gctcgatgga ggatacccat acgatgttcc agattacgct gctcgaggtt
      121 atcaaacaag tttgtacaaa aaagttggca tgccgctcta ctccgttact gtaaaatggg
      181 gaaaggagaa atttgaaggt gtagaattga atacagatga acctccaatg gtattcaagg
      241 ctcagctgtt tgcgttgact ggagtccagc ctgccagaca gaaagttatg gtgaaaggag
      301 gaacgctaaa ggatgatgat tggggaaaca tcaaaataaa aaatggaatg actctactaa
      361 tgatggggtc agcagatgct cttccagaag aaccctcagc caaaactgtc ttcgtagaag
      421 acatgacaga agaacagtta gcatctgcta tggagttacc atgtggattg acaaaccttg
      481 gtaacacttg ttacatgaat gccacagttc agtgtattcg ttctgtgcct gaactcaaag
      541 atgcccttaa aaggtatgca ggtgccttga gagcttcagg ggaaatggct tcagcgcagt
      601 atattactgc agcccttaga gatttgtttg attccatgga taaaacttct tccagtattc
      661 cacctattat tctactgcag tttttgcaca tggctttccc acagtttgcc gagaaaggtg
      721 aacaaggaca gtatcttcaa caggatgcta atgaatgttg gatacaaatg atgcgagtat
      781 tgcaacagaa attggaagca atagaggatg attctgttaa agagacagac tcctcatctg
      841 catcggcagc gacaccttct aaaaagaaaa gtttaatcga tcagttcttc ggtgttgagt
      901 ttgaaactac catgaaatgt acagaatctg aagaagaaga agtc
//
