LOCUS       sequence-153353-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human NEUROGENIN2 (hNGN2) and puromycin resistance gene 
            under control of TetON promoter. This lentiviral vector is used to 
            generate NGN2-iNs (induced neurons) from hiPSCs and hiPSC-NPCs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Ho SM, Hartley BJ, Tcw J, Beaumont M, Stafford K, Slesinger PA, 
            Brennand KJ
  TITLE     Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural
            progenitor cells.
  JOURNAL   Methods. 2015 Nov 25. pii: S1046-2023(15)30159-6. doi: 
            10.1016/j.ymeth.2015.11.019.
  PUBMED    26626326
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            2015 Nov 25. pii: S1046-2023(15)30159-6. doi: 
            10.1016/j.ymeth.2015.11.019."
COMMENT     Sequence Label: pLV-TetO-hNGN2-Puro
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(62..655)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="EYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVT
                     ELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQ
                     QQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAP
                     RNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(145..165)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             complement(668..724)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 attttgtaat ccagaggttg attatcgata agcttgatat cgaattagct agcttaatta
       61 atcaggcacc gggcttgcgg gtcatgcacc aggtgcgcgg tccttcgggc acctcgacgt
      121 cggcggtgac ggtgaagccg agccgctcgt agaaggggag gttgcggggc gcggaggtct
      181 ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac tccggggagc acgacggcgc
      241 tgcccagacc cttgccctgg tggtcgggcg agacgccgac ggtggccagg aaccacgcgg
      301 gctccttggg ccggtgcggc gccaggaggc cttccatctg ttgctgcgcg gccagccggg
      361 aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa caccgccccc gcttcgacgc
      421 tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc cgcgacccac accttgccga
      481 tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc ggtgacccgc tcgatgtggc
      541 ggtccggatc gacggtgtgg cgcgtggcgg ggtagtcggc gaacgcggcg gcgagggtgc
      601 gtacggccct ggggacgtcg tcgcgggtgg cgaggcgcac cgtgggcttg tactctctag
      661 acaccatagg accagggttt tcctcgacat caccggcctg cttgagtagc gagaaattcg
      721 tggcgccaga tccgaattcg atacaatccc tggctatggg gaggtgaggt gcatagcggt
      781 gcttgtcggg aggtgggggc tgccaatagt ccatgtctga cccggccggg ctggcgggcg
      841 ataaagtgca gctgtagg
//
