LOCUS       sequence-153354-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human expression plasmid for SpCas9-HF4 variant: 
            CMV-T7-humanSpCas9-HF4(Y450A, N497A, R661A, Q695A, 
            Q926A)-NLS-3xFLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Kleinstiver BP, Pattanayak V, Prew MS, Tsai SQ, Nguyen NT, Zheng Z, 
            Keith Joung J
  TITLE     High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide 
            off-target effects.
  JOURNAL   Nature. 2016 Jan 6. doi: 10.1038/nature16526.
  PUBMED    26735016
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Jan 6. doi: 10.1038/nature16526."
COMMENT     Sequence Label: MSP2133
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aagcagcgga ctttcgacaa cggtagcatt ccacatcaaa tccacttagg cgaattgcat
       61 gctatactta gaaggcagga ggatttttat ccgttcctca aagacaatcg tgaaaagatt
      121 gagaaaatcc taacctttcg catacctgcc tatgtgggac ccctggcccg agggaactct
      181 cggttcgcat ggatgacaag aaagtccgaa gaaacgatta ctccctggaa ttttgaggaa
      241 gttgtcgata aaggtgcgtc agctcaatcg ttcatcgaga ggatgaccgc ctttgacaag
      301 aatttaccga acgaaaaagt attgcctaag cacagtttac tttacgagta tttcacagtg
      361 tacaatgaac tcacgaaagt taagtatgtc actgagggca tgcgtaaacc cgcctttcta
      421 agcggagaac agaagaaagc aatagtagat ctgttattca agaccaaccg caaagtgaca
      481 gttaagcaat tgaaagagga ctactttaag aaaattgaat gcttcgattc tgtcgagatc
      541 tccggggtag aagatcgatt taatgcgtca cttggtacgt atcatgacct cctaaagata
      601 attaaagata aggacttcct ggataacgaa gagaatgaag atatcttaga agatatagtg
      661 ttgactctta ccctctttga agatcgggaa atgattgagg aaagactaaa aacatacgct
      721 cacctgttcg acgataaggt tatgaaacag ttaaagaggc gtcgctatac gggctgggga
      781 gccttgtcgc ggaaacttat caacgggata agagacaagc aaagtggtaa aactattctc
      841 gattttctaa agagcgacgg cttcgccaat aggaacttta tggccctgat ccatgatgac
      901 tctttaacct tcaaagagga tatacaaaag gcacaggttt ccggacaagg ggactcattg
      961 cacgaacata t
//
