LOCUS       sequence-153360-         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of shRNA for mouse Tet3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Montagner S, Leoni C, Emming S, Della Chiara G, Balestrieri C, 
            Barozzi I, Piccolo V, Togher S, Ko M, Rao A, Natoli G, Monticelli S
  TITLE     TET2 Regulates Mast Cell Differentiation and Proliferation through 
            Catalytic and Non-catalytic Activities.
  JOURNAL   Cell Rep. 2016 May 17;15(7):1566-79. doi: 
            10.1016/j.celrep.2016.04.044. Epub 2016 May 5.
  PUBMED    27160912
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.04.044"; journalName: "Cell Rep"; date: 
            "2016-05-17- 17"; volume: "15"; issue: "7"; pages: "1566-79"
COMMENT     Sequence Label: pAGM_Puro_shRNA n.1_mTet3
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           91..212
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           238..252
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           278..404
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(535..555)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 agacgcgcac ctggtgcatg acccgcaagc ccggtgcctg agcggccgca agccttgtta
       61 agtgctcgct tcggcagcac atatactatg ttgaatgagg cttcagtact ttacagaatc
      121 gttgcctgca catcttggaa acacttgctg ggattacttc ttcaggttaa cccaacagaa
      181 ggctcgagaa ggtatattgc tgttgacagt gagcggcctg ttaggcagat tgttctagtg
      241 aagccacaga tgtagaacaa tctgcctaac aggctgccta ctgcctcgga attcaagggg
      301 ctactttagg agcaattatc ttgtttacta aaactgaata ccttgctatc tctttgatac
      361 atttttacaa agctgaatta aaatggtata aattaaatca cttttttcaa ttggaagact
      421 aatgcgttta aacacgcggc gacgcgtatc gataccggtg gcccctgggg ccgcgatcgc
      481 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
      541 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
      601 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
      661 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
      721 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
      781 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
      841 gttgggcact gacaattcc
//
