LOCUS       sequence-153362-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Low affinity fluorescent calcium indicator of the GAP (GFP-Aequorin 
            Protein) family targeted to the endoplasmic reticulum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Navas-Navarro P, Rojo-Ruiz J, Rodriguez-Prados M, Ganfornina MD, 
            Looger LL, Alonso MT, Garcia-Sancho J
  TITLE     GFP-Aequorin Protein Sensor for Ex Vivo and In Vivo Imaging of 
            Ca(2+) Dynamics in High-Ca(2+) Organelles.
  JOURNAL   Cell Chem Biol. 2016 Jun 1. pii: S2451-9456(16)30163-5. doi: 
            10.1016/j.chembiol.2016.05.010.
  PUBMED    27291400
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Chem 
            Biol. 2016 Jun 1. pii: S2451-9456(16)30163-5. doi: 
            10.1016/j.chembiol.2016.05.010."
COMMENT     Sequence Label: pcDNA3_erGAP3
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctccaa agcttccgcc accatgctcc tctctgtgcc cctcctcctc ggtctcctgg
      121 gactggccgt agccaatgac gcctcaaaag gcgaagagct gttcacaggc gtggtcccta
      181 tccaggtgga actcgatgga gacgtgaacg gccataaatt ctccgtatca ggcgaggggg
      241 aaggagacgc cacatatgga aagctgacac tcaaatttat ctgcaccaca ggaaaactgc
      301 ctgtcccatg gccaaccctc gtgacaacct tctcatacgg cgtgcaatgt tttagcagat
      361 atccagacca catgaaaagg catgacttct ttaagagcgc aatgccagaa ggatacgtcc
      421 aggagaggac cattagcttc aaggacgatg gcaattataa gacaagagcc gaggtcaaat
      481 tcgagggaga cactctcgtg aacagaattg aactgaaagg gatcgatttt aaagaagatg
      541 gcaacatcct cggacataag ctcgagtaca actacaactc tcataatgtg tacataaccg
      601 ccgacaaaca gaagaacggg atcaaagcta atttcaaggt gaggcacaac atagaagacg
      661 gcggcgtaca gctggcttac cactatcagc agaacacacc cataggggac ggtcccgtgc
      721 tcctgccaga taatcactat ctgtccacac aatcagccct gagcaaggac cctaacgaga
      781 aaagagacca tatgg
//
