LOCUS       sequence-153374-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express rhesus TRIM5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Li YL, Chandrasekaran V, Carter SD, Woodward CL, Christensen DE, 
            Dryden KA, Pornillos O, Yeager M, Ganser-Pornillos BK, Jensen GJ, 
            Sundquist WI
  TITLE     Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids.
  JOURNAL   Elife. 2016 Jun 2;5. pii: e16269. doi: 10.7554/eLife.16269.
  PUBMED    27253068
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-06-2"; volume: "5. pii: e16269. doi"; pages: " 
            10.7554/eLife.16269"
COMMENT     Sequence Label: CSII-IDR2-rhesusTRIM5-FOS
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    67..119
                     /label=MCS
                     /note="multiple cloning site"
     CDS             complement(123..146)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(153..176)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 acgcacaccg gccttattcc aagcggcttc ggccagtaac gttagggggg ggggagggag
       61 aggggcggat cccgggcccg cggtaccgtc gactgcagaa ttcgaagctt gagctcgagt
      121 tatttttcga actgcgggtg gctccaagcg ctcttgtcat cgtcatcctt gtaatcgaat
      181 ccggatccgg taccagagct tggtgagcac agggtcatgg ggactgtaca ttttctggga
      241 tttaaatatg gaaatacagg cttagaaaaa gaacactgag aaaacttata gatgagaaat
      301 ccatggtttg tgatattgaa gaatgagaca gtgcaagcct catagtctac gaaaactcca
      361 acacgatcag gacaaataat cacagagagg ggcacaatga aaggagcaaa aggagtatgt
      421 gaggaaccat cctggaaaac actatattta actccttcct gtaaccctat aacccagtag
      481 ccatatttag gttgataatt ttcattttgt tcaatattat acattgcatc ggattggaag
      541 ccagcacata cccccaggat ccaagcactt ttcttggaca catctacctc ccagtaatgc
      601 ttccctgatg tgatactttg ggagcccagg atgccagtac aataattgaa attcgtgagt
      661 gacggaaacg taaataatgt ccctggtgcc tgatacatta tctgtgggtt ccgagagctc
      721 acttgtctct tatcttcagc aatgacagca tgcgaaatgt tgtttgtagc cagtgtcaca
      781 tcaacccagt agcgtcgggc atctgttagc tctctaaaca tgtctagcat tcctttcaga
      841 tcag
//
