LOCUS       sequence-153378-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculoviral entry vector to produce Bac-to-bac baculoviruses.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Li YL, Chandrasekaran V, Carter SD, Woodward CL, Christensen DE, 
            Dryden KA, Pornillos O, Yeager M, Ganser-Pornillos BK, Jensen GJ, 
            Sundquist WI
  TITLE     Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids.
  JOURNAL   Elife. 2016 Jun 2;5. pii: e16269. doi: 10.7554/eLife.16269.
  PUBMED    27253068
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-06-2"; volume: "5. pii: e16269. doi"; pages: " 
            10.7554/eLife.16269"
COMMENT     Sequence Label: pFastBac1-Kozak-rhTRIM5alpha-PFOS
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(214..237)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(274..297)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(304..327)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(343..366)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
       61 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc tctagtactt
      121 ctcgacaagc ttggtaccgc atgcctcgag actgcaggct ctagattcga aagcggccgc
      181 gactagtgag ctcgtcgacg taggtcagct agctttctcg aactgcgggt ggctccacga
      241 tccacctccc gatccacctc gggcacctcc acctttttcg aactgcgggt ggctccagaa
      301 tcccttgtca tcgtcatcct tgtaatccgg agatcttgta ccggggccct ggaacaggac
      361 ttccagagag cttggtgagc acagagtcat ggggactgta cattttctgg gatttaaata
      421 tggaaataca ggcttagaaa aagaacactg agaaaactta tagatgagaa atccatggtt
      481 tgtgatattg aagaatgaga cagtgcaagc ctcatagtct acgaaaactc caacacgatc
      541 aggacaaata atcacagaga ggggcacaat gaaaggagca aaaggagtat gtgaggaacc
      601 atcctggaaa acactatatt taactccttc ctgtaaccct ataacccagt agccatattt
      661 aggttgataa ttttcatttt gttcaatatt atacattgca tcggattgga agccagcaca
      721 tacccccagg atccaagcac ttttcttgga cacatctacc tcccagtaat gcttccctga
      781 tgtgatactt tgggagccca ggacgccagt acaataattg aaattcgtga gtgacggaa
//
