LOCUS       sequence-153379-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Low affinity fluorescent calcium indicator of the GAP (GFP-Aequorin 
            Protein) family targeted to the endoplasmic reticulum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  TITLE     pcDNA3_erGAP2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3_erGAP2
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(140..142,143..853)
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="ASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(170..198)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     793..813
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctccaa agcttcgcca ccatgctgct gagcgtgccc ctgctgctgg gcctgctggg
      121 cctggccgtg gccaacgatg ctagcaaagg agaagaactt ttcactggag ttgtcccaat
      181 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
      241 aggtgatgct acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
      301 tgttccatgg ccaacacttg tcactacttt ctcttatggt gttcaatgct tttcccgtta
      361 tccggatcat atgaaacggc atgacttttt caagagtgcc atgcccgaag gttatgtaca
      421 ggaacgcact atatctttca aagatgacgg gaactacaag acgcgtgctg aagtcaagtt
      481 tgaaggtgat acccttgtta atcgtatcga gttaaaaggt attgatttta aagaagatgg
      541 aaacattctc ggacacaaac tcgagtacaa ctataactca cacaatgtat acatcacggc
      601 agacaaacaa aagaatggaa tcaaagctaa cttcaaaatt cgccacaaca ttgaagatgg
      661 atccgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
      721 tttaccagac aaccattacc tgtcgacaca atctgccctt tcgaaagatc ccaacgaaaa
      781 gcgtgaccac atggtccttc ttgagtttgt aactgctgct gggattacac atggcatgga
      841 tgagctctac aaacc
//
