LOCUS       sequence-153382-         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent Reporter for Dopaminergic Neuron Differentiation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Oh MS, Hong SJ, Huh Y, Kim KS
  TITLE     Expression of transgenes in midbrain dopamine neurons using the 
            tyrosine hydroxylase promoter.
  JOURNAL   Gene Ther. 2009 Mar;16(3):437-40. doi: 10.1038/gt.2008.148. Epub 
            2008 Sep 18.
  PUBMED    18800154
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/gt.2008.148"; journalName: "Gene Ther"; date: "2009-03"; 
            volume: "16"; issue: "3"; pages: "437-40"
COMMENT     Sequence Label: pAAV2.5-THP-dsRed2
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    66..290
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     279..296
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(304..981)
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(366..389)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     781..801
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 ggccaggaga ggcactgggg aggggtcaca gggatgccac ccgtagatct ctcgaccctc
       61 agaagccata gagcccaccg catccccagc atgcctgcta ttgtcttccc aatcctcccc
      121 cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca gacaatgcga
      181 tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca gggtcaagga
      241 aggcacgggg gaggggcaaa caacagatgg ctggcaacta gaaggcacag tcgaggctct
      301 agattacagg aacaggtggt ggcggccctc ggtgcgctcg tactgctcca cgatggtgta
      361 gtcctcgttg tgggaggtga tgtccagctt ggcgtccacg tagtagtagc cgggcagctg
      421 cacgggcttc ttggccatgt agatggactt gaactccacc aggtagtggc cgccgtcctt
      481 cagcttcagg gccttgtggg tctcgccctt cagcacgccg tcgcgggggt acaggcgctc
      541 ggtggaggcc tcccagccca tggtcttctt ctgcatcacg gggccgtcgg aggggaagtt
      601 cacgccgatg aacttcacct tgtagatgaa gcagccgtcc tgcagggagg agtcctgggt
      661 cacggtcgcc acgccgccgt cctcgaagtt catcacgcgc tcccacttga agccctcggg
      721 gaaggacagc ttcttgtagt cggggatgtc ggcggggtgc ttcacgtaca ccttggagcc
      781 gtactggaac tggggggaca ggatgtccca ggcgaagggc agggggccgc ccttggtcac
      841 cttcagcttc acggtgttgt ggccctcgta ggggcggccc tcgccctcgc cctcgatctc
      901 gaactcgtgg ccgttcacgg tgccctccat gcgcaccttg aagcgcatga actcggtgat
      961 gacgttctcg gaggaggcca tggccaggtc ctct
//
