LOCUS       sequence-153384-         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Ca2+ sensor GAP in the cytosol.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Rodriguez-Garcia A, Rojo-Ruiz J, Navas-Navarro P, Aulestia FJ, 
            Gallego-Sandin S, Garcia-Sancho J, Alonso MT
  TITLE     GAP, an aequorin-based fluorescent indicator for imaging Ca2+ in 
            organelles.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Feb 18;111(7):2584-9. doi: 
            10.1073/pnas.1316539111. Epub 2014 Feb 5.
  PUBMED    24501126
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1316539111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-02-18- 18"; volume: "111"; issue: "7"; pages: "2584-9"
COMMENT     Sequence Label: pcDNA3.cytGAP
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(165..183)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctccaa agcttggcat tccggtactg ttggtaaaat ggaagacgcc aaaaacataa
      121 agaaaggccc ggcgccattc tatcctctag aggatggaac cgctggagag caactgcata
      181 aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg
      241 aggtgaacat cacgtacgcg gaatacttcg aaatgtccgt tcggttggca gaagctatga
      301 aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat
      361 tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca
      421 tttataatga acgtgaattg ctcaacagta tgaacatttc gcagcctacc gtagtgtttg
      481 tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaaattacca ataatccaga
      541 aaattattat catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg
      601 tcacatctca tctacctccc ggttttaatg aatacgattt tgtaccagag tcctttgatc
      661 gtgacaaaac aattgcactg ataatgaatt cctctggatc tactgggtta cctaagggtg
      721 tggcccttcc gcatagaact gcctgcgtca gattctcgca tgccagagat cctatttttg
      781 gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat cacggttttg
      841 gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat
      901 ttgaagaaga gctgttttta cgatcccttc aggattacaa aattcaa
//
