LOCUS       sequence-153385-         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Ca2+ sensor GAP in the cytosol.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Rodriguez-Garcia A, Rojo-Ruiz J, Navas-Navarro P, Aulestia FJ, 
            Gallego-Sandin S, Garcia-Sancho J, Alonso MT
  TITLE     GAP, an aequorin-based fluorescent indicator for imaging Ca2+ in 
            organelles.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Feb 18;111(7):2584-9. doi: 
            10.1073/pnas.1316539111. Epub 2014 Feb 5.
  PUBMED    24501126
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1316539111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-02-18- 18"; volume: "111"; issue: "7"; pages: "2584-9"
COMMENT     Sequence Label: pcDNA3.cytGAP
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(726..746)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 aatagggccc tctagatgca tgctcgagcg gccgccagtg tgatggatat ctgcagaatt
       61 cttaggggac agctccaccg tagagctttt cgcaagcagg atccatggtg taccaaaatc
      121 ctaaatgttg tcttgtcatc tcatcaacat cgagttgtcc actttcatca atatcgcaca
      181 ctctgaatgt ttcctcgcaa tcttctgatg attggatgat accagcagct ttggtgtatg
      241 ctttccattc atccagtgta atggctccat tttgatcttt gtcaacgata tcaaacaaag
      301 catcacccca tatacggatg agcgttggtt cgtttttggc gtatttctcc aattcatcag
      361 tagccaattt tttccatcct tcaatatatg caggccaatc agtttccaca ccatatttca
      421 ttccagctcc tccgaagaag gcttctacag catctttgtg tcgtttggct tgctcaggtg
      481 ttgctccaag gttattgatg acaatatcag atgccttgta gaccatctcg tcaagagaga
      541 tttttccatt gtggttgaca tcaaggaaat tgaacatatg cttgtgtcgt ccaatccatc
      601 ttgggttgtc gaagtctgat gtaagtttac cggtacccgc ggtcggtgca gtggtcggcg
      661 tggttgctgg ggtcgctgta cccggtttgt agagctcatc catgccatgt gtaatcccag
      721 cagcagttac aaactcaaga aggaccatgt ggtcacgctt ttcgttggga tctttcgaaa
      781 gggcagattg tgtcgacagg taatggttgt ctggtaaaag gacagggcca tcgccaattg
      841 gagtattttg ttgataatgg tctgctagtt gaa
//
