LOCUS       sequence-153396-         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Ca2+ sensor GAP in the nucleus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Rodriguez-Garcia A, Rojo-Ruiz J, Navas-Navarro P, Aulestia FJ, 
            Gallego-Sandin S, Garcia-Sancho J, Alonso MT
  TITLE     GAP, an aequorin-based fluorescent indicator for imaging Ca2+ in 
            organelles.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Feb 18;111(7):2584-9. doi: 
            10.1073/pnas.1316539111. Epub 2014 Feb 5.
  PUBMED    24501126
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1316539111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-02-18- 18"; volume: "111"; issue: "7"; pages: "2584-9"
COMMENT     Sequence Label: pcDNA3.nucGAP
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    70..126
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        133..151
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             618..665
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc naaattaata cgactcacta tagggagacc
       61 caagctccaa agcttgcatg cctgcaggtc gactctanag gatccccggg taccgagctc
      121 gaattcccca cctaatacga ctcactatag ggagaatggc ctctacagtg agcaacacca
      181 gcaaacttga gaaacctgtg tcccttatat gggggtgtga actgaatgag caaaacaaga
      241 cgtttgagtt taaggtanaa gatgatgagg aaaaatgtga gcatcagttg gcgttgcgca
      301 cggtgtgtct gggggacaag gcaaaggatg agttccacat tgtagaaatc gttacacaag
      361 aggagggagc ggaaaaatct gttccaattg ccactctaaa gccttctatt ctacccatgg
      421 caactatggt gggcattgag ctgactcctc cagttacttt ccggttaaaa gctggttccg
      481 gcccactgta catcantggt caacacgtag cgatggagga agattactca tgggcagaag
      541 aggaagatga aggggaagct gaaggagaag aggaggaaga agaggaagaa gatcaagaat
      601 ctccacccaa agctgtaaag aggcctgcgg ctaccaaaaa agcaggccag gcaaagaaga
      661 agaaacttga caaanaggac gagagctccg aggaagacag tccaaccaaa aagggcaaag
      721 gagccggaag aggaa
//
