LOCUS       sequence-153400-         687 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Ca2+ sensor GAP1 in the trans Golgi apparatus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Rodriguez-Garcia A, Rojo-Ruiz J, Navas-Navarro P, Aulestia FJ, 
            Gallego-Sandin S, Garcia-Sancho J, Alonso MT
  TITLE     GAP, an aequorin-based fluorescent indicator for imaging Ca2+ in 
            organelles.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Feb 18;111(7):2584-9. doi: 
            10.1073/pnas.1316539111. Epub 2014 Feb 5.
  PUBMED    24501126
REFERENCE   2  (bases 1 to 687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1316539111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-02-18- 18"; volume: "111"; issue: "7"; pages: "2584-9"
COMMENT     Sequence Label: pcDNA3.tgoGAP1
FEATURES             Location/Qualifiers
     source          1..687
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     43..62
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      341..350
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(380..408)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag
       61 ggagacccaa gctccaaagc ttgccaccat gaggcttcgg gagccgctcc tgagcggcag
      121 cgccgcgatg ccaggcgcgt ccctacagcg ggcctgccgc ctgctcgtgg ccgtctgcgc
      181 tctgcacctt ggcgtcaccc tcgtttacta cctggctggc cgcgacctga gccgcctgcc
      241 ccaactggtc ggagtctcca caccgctgca gggcggctcg aacagtgccg ccgccatcgg
      301 gcagtcctcc ggggagctcc ggaccggagg ggccaagctt gccaccatgg ctagcaaagg
      361 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg
      421 gcacaaattt tctgtcagtg gagagggtga aggtgatgct acatacggaa aacttaccct
      481 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt
      541 ctcttatggt gttcaatgct tttcccgtta tccggatcat atgaaacggc atgacttttt
      601 caagagtgcc atgcccgaag gttatgtaca ggaacgcact atatctttca aagatgacgg
      661 gaactacaag acgcgtgctg aagtcaa
//
