LOCUS       sequence-153407-         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Negative control shRNA vector containing non-hairpin insert..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Moffat J, Grueneberg DA, Yang X, Kim SY, Kloepfer AM, Hinkle G, 
            Piqani B, Eisenhaure TM, Luo B, Grenier JK, Carpenter AE, Foo SY, 
            Stewart SA, Stockwell BR, Hacohen N, Hahn WC, Lander ES, Sabatini 
            DM, Root DE
  TITLE     A lentiviral RNAi library for human and mouse genes applied to an 
            arrayed viral high-content screen.
  JOURNAL   Cell. 2006 Mar 24. 124(6):1283-98.
  PUBMED    16564017
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2006 
            Mar 24. 124(6):1283-98."
COMMENT     Sequence Label: pLKO.1 - TRC control
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     109..128
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    249..366
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag
       61 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
      121 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
      181 aacaccggtc cgcaggtatg cacgcgtgaa ttctcgacct cgagacaaat ggcagtattc
      241 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
      301 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
      361 aattttcggg tttattacag ggacagcaga gatccacttt ggccgcggct cgagggggtt
      421 ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg
      481 tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg
      541 cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc
      601 gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc
      661 cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg
      721 accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga gcagcggccg
      781 g
//
