LOCUS       sequence-153410-         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Adeno-associated virus plasmid, Cre-dependent FLEX switch, mAPX.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Joesch M, Mankus D, Yamagata M, Shahbazi A, Shalek R, Suissa-Peleg 
            A, Meister M, Lichtman JW, Scheirer WJ, Sanes JR
  TITLE     Reconstruction of genetically identified neurons imaged by 
            serial-section electron microscopy.
  JOURNAL   Elife. 2016 Jul 7;5. pii: e15015. doi: 10.7554/eLife.15015.
  PUBMED    27383271
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-07-7"; volume: "5. pii: e15015. doi"; pages: " 
            10.7554/eLife.15015"
COMMENT     Sequence Label: AAV-CAG-DIO-mAPX
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(43..76)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(127..160)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 gtaatccaga ggttgattat cgataagctt gatatcgaat tcataacttc gtatagcata
       61 cattatacga agttattctt tgcaccattc taaagaataa cagtgataat ttctgggtta
      121 aggcaaataa cttcgtatag gatactttat acgaagttat gctagccgcc accatgaggg
      181 gaaagtcata cccaaccgtg agcccagatt accagaaggc tattgagaag gcaaaaagga
      241 aactgagggg attcatcgca gaaaagaaat gcgccccact gatcctgcga ctggctttcc
      301 acagcgcagg aacatttgac tccaagacca aaacaggcgg accctttggg actatcaagc
      361 accaggctga actggcacat ggggccaaca atggcctgga cattgctgtg agactgctgg
      421 agccaatcaa agaacagttc cccattgtct cctacgcaga cttctaccag ctggccggcg
      481 tggtcgctgt ggagattacc ggcggccccg aggtcccttt ccacccagga agggaggaca
      541 aacctgaacc ccctccagag gggagactgc cagatgctac caagggcagc gaccatctgc
      601 gggacgtgtt cggaaaagca atggggctgt ctgaccagga tatcgtggcc ctgagtggag
      661 ggcacaccat tggcgccgct cataaggaac gctcaggctt cgagggaccc tggacaagca
      721 accctctgat cttcgacaat tcctacttta ctgaactgct gaccggcgag aaggatggac
      781 tgctgcagct gccctctgac aaagccctgc tgacagatag cgtgttcagg cctctggtcg
      841 aaaagtatgc agccgacgag gatgtgttct tcgccgacta tgctgaggca cacctgaa
//
