LOCUS       sequence-153414-       10657 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10657)
  AUTHORS   Salmon P, Oberholzer J, Occhiodoro T, Morel P, Lou J, Trono D
  TITLE     Reversible immortalization of human primary cells by 
            lentivector-mediated transfer of specific genes.
  JOURNAL   Mol Ther. 2000 Oct . 2(4):404-14.
  PUBMED    11020357
REFERENCE   2  (bases 1 to 10657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2000 Oct . 2(4):404-14."
COMMENT     Sequence Label: pLOX-gfp-iresTK
FEATURES             Location/Qualifiers
     source          1..10657
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             4..637
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    684..809
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        2043..2346
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2347..2550
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2501..2521
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2547..2571
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2651..2660
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2657..2659,2660..2662,2663..3373)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2657..2659,2660..2662,2663..3373)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2702..2723)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2963..2982)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    3469..3930
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3525..3542)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3752..3771
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3935..5065
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     protein_bind    complement(5221..5254)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             5274..5454
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5479..5497)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5480..5497)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5787..5806)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6020..6039)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6139..6161
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6199..6217)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6285..6389
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6390..6458,6459..7250)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6608..6627)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7421..8009
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7910..7929
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8163..8180
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8250..8270)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8255..8584
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8435..8570
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9718..9783
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9913..9933
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10358..10492
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10395..10414)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10449..10468
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc
       61 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
      121 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
      181 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
      241 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
      301 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
      361 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
      421 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
      481 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      541 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      601 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      661 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      721 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      781 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      841 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      901 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      961 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     1021 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     1081 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     1141 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgataagc ttgggagttc
     2041 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     2101 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     2161 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     2221 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     2281 tacatgacct tacgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     2341 accatggtga tgcggttttg gcagtacacc aatgggcgtg gatagcggtt tgactcacgg
     2401 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     2461 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     2521 gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga
     2581 cgccatccac gctgttttga cctccataga agacaccgac tctagctaga ggatcctagc
     2641 gctaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     2701 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     2761 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     2821 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     2881 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     2941 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     3001 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     3061 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     3121 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     3181 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     3241 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     3301 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     3361 cgagctgtac aagtccggac tcagatctcg agctcaagct tcgaattaat tctgcagtcg
     3421 acattaattc cggttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     3481 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     3541 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     3601 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     3661 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     3721 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     3781 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
     3841 atgctttaca tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa ccacggggac
     3901 gtggttttcc tttgaaaaac acgatgataa taccatggct tcgtacccct gccatcaaca
     3961 cgcgtctgcg ttcgaccagg ctgcgcgttc tcgcggccat agcaaccgac gtacggcgtt
     4021 gcgccctcgc cggcagcaag aagccacgga agtccgcctg gagcagaaaa tgcccacgct
     4081 actgcgggtt tatatagacg gtcctcacgg gatggggaaa accaccacca cgcaactgct
     4141 ggtggccctg ggttcgcgcg acgatatcgt ctacgtaccc gagccgatga cttactggca
     4201 ggtgctgggg gcttccgaga caatcgcgaa catctacacc acacaacacc gcctcgacca
     4261 gggtgagata tcggccgggg acgcggcggt ggtaatgaca agcgcccaga taacaatggg
     4321 catgccttat gccgtgaccg acgccgttct ggctcctcat atcggggggg aggctgggag
     4381 ctcacatgcc ccgcccccgg ccctcaccct catcttcgac cgccatccca tcgccgccct
     4441 cctgtgctac ccggccgcgc gataccttat gggcagcatg accccccagg ccgtgctggc
     4501 gttcgtggcc ctcatcccgc cgaccttgcc cggcacaaac atcgtgttgg gggcccttcc
     4561 ggaggacaga cacatcgacc gcctggccaa acgccagcgc cccggcgagc ggcttgacct
     4621 ggctatgctg gccgcgattc gccgcgttta cgggctgctt gccaatacgg tgcggtatct
     4681 gcagggcggc gggtcgtggc gggaggattg gggacagctt tcggggacgg ccgtgccgcc
     4741 ccagggtgcc gagccccaga gcaacgcggg cccacgaccc catatcgggg acacgttatt
     4801 taccctgttt cgggcccccg agttgctggc ccccaacggc gacctgtaca acgtgtttgc
     4861 ctgggccttg gacgtcttgg ccaaacgcct ccgtcccatg cacgtcttta tcctggatta
     4921 cgaccaatcg cccgccggct gccgggacgc cctgctgcaa cttacctccg ggatggtcca
     4981 gacccacgtc accacccccg gctccatacc gacgatctgc gacctggcgc gcacgtttgc
     5041 ccgggagatg ggggaggcta actgaaacac ggaaggggta ccgaattcct cgacggtacc
     5101 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     5161 gggactggaa gggctaattc actcccaacg aagacaagat gggatcaatt caccatggga
     5221 ataacttcgt atagcataca ttatacgaag ttatgctgct ttttgcttgt actgggtctc
     5281 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     5341 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     5401 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatct
     5461 agaattaatt ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt
     5521 tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt gtgtagcatc
     5581 tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt
     5641 tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc
     5701 aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac
     5761 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
     5821 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
     5881 ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct
     5941 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg
     6001 tcgaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     6061 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     6121 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     6181 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     6241 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     6301 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     6361 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     6421 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     6481 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     6541 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     6601 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     6661 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     6721 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     6781 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     6841 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     6901 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     6961 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     7021 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     7081 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     7141 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     7201 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     7261 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     7321 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7381 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     7441 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     7501 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     7561 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     7621 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     7681 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7741 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     7801 tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7861 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7921 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7981 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     8041 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     8101 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     8161 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     8221 cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     8281 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     8341 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     8401 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     8461 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     8521 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     8581 caaaaagctt ggacacaaga caggcttgcg agatatgttt gagaatacca ctttatcccg
     8641 cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg
     8701 cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag
     8761 ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc
     8821 agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta
     8881 ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac tgggtattcg
     8941 tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg agcttaaagt
     9001 gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc tggtggatac
     9061 cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt
     9121 cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc cgcaagatac
     9181 ctggattgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta
     9241 atcttttcaa cgcctggcac tgccgggcgt tgttcttttt aacttcaggc gggttacaat
     9301 agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc
     9361 tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc tttaatcacg
     9421 gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac catccctcac tggtatcgca
     9481 tgattaaccg tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc
     9541 aggcacgtat tgtgatgagc gatgccgaac gtaccgacga tgatttatac gatacggtga
     9601 ttggctaccg tggcggcaac tggatttatg agtgggcccc ggatctttgt gaaggaacct
     9661 tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta
     9721 aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt
     9781 tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa
     9841 aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa
     9901 cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa
     9961 ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt
    10021 tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta
    10081 acctttataa gtaggcataa cagttataat cataacatac tgttttttct tactccacac
    10141 aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta
    10201 atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat
    10261 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
    10321 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
    10381 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    10441 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
    10501 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
    10561 ttacctagtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa
    10621 gaaattgtat ttgttaaata tgtactacaa acttagt
//
