LOCUS       sequence-153420-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of shRNA for mouse Tet3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Montagner S, Leoni C, Emming S, Della Chiara G, Balestrieri C, 
            Barozzi I, Piccolo V, Togher S, Ko M, Rao A, Natoli G, Monticelli S
  TITLE     TET2 Regulates Mast Cell Differentiation and Proliferation through 
            Catalytic and Non-catalytic Activities.
  JOURNAL   Cell Rep. 2016 May 17;15(7):1566-79. doi: 
            10.1016/j.celrep.2016.04.044. Epub 2016 May 5.
  PUBMED    27160912
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.04.044"; journalName: "Cell Rep"; date: 
            "2016-05-17- 17"; volume: "15"; issue: "7"; pages: "1566-79"
COMMENT     Sequence Label: pAGM_Puro_shRNA n.2_mTet3
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           87..208
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           234..248
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           274..400
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(531..551)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(950..961)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgagcg gccgcaagcc ttgttaagtg
       61 ctcgcttcgg cagcacatat actatgttga atgaggcttc agtactttac agaatcgttg
      121 cctgcacatc ttggaaacac ttgctgggat tacttcttca ggttaaccca acagaaggct
      181 cgagaaggta tattgctgtt gacagtgagc ggctccaacg agaagctatt ttagtgaagc
      241 cacagatgta aaatagcttc tcgttggagc tgcctactgc ctcggaattc aaggggctac
      301 tttaggagca attatcttgt ttactaaaac tgaatacctt gctatctctt tgatacattt
      361 ttacaaagct gaattaaaat ggtataaatt aaatcacttt tttcaattgg aagactaatg
      421 cgtttaaaca cgcggcgacg cgtatcgata ccggtggccc ctggggccgc gatcgctaat
      481 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
      541 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
      601 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
      661 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
      721 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
      781 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
      841 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
      901 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccctt cggccctcaa
      961 tccagcggaa c
//
