LOCUS       sequence-153432-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Supplemental Figure 3. Integration Site 1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP74
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(78..111)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    776..823
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
ORIGIN
        1 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
       61 tgcatgagat cagatctgaa gttcctatac tttctagaga ataggaactt caagcttgtg
      121 gaacattgag accaaacaag actcgcttcg atgctttcag atccattttc ccagcaggta
      181 ccgtctccgg tgctccgaag gtaagagcaa tgcaactcat aggagaattg gaaggagaaa
      241 agagaggtgt ttatgcgggg gccgtaggac actggtcgta cgatggaaaa tcgatggaca
      301 catgtattgc cttaagaaca atggtcgtca aggacggtgt cgcttacctt caagccggag
      361 gtggaattgt ctacgattct gacccctatg acgagtacat cgaaaccatg aacaaaatga
      421 gatccaacaa taacaccatc ttggaggctg agaaaatctg gaccgatagg ttggccagag
      481 acgagaatca aagtgaatcc gaagaaaacg atcaatgaac ggaggacgta agtaggaatt
      541 tatgtaatca tgccaataca tctttagatt tcttcctctt ctttttcatg agattattgg
      601 aaaccaccag aatcgaatat aaaaggcgaa cacctttccc aattttggtt tctcctgacc
      661 caaagacttt aaatttaatt tatttgtccc tatttcaatc aattgaacaa ctatttcggc
      721 tggacggcga cgtaaacggc cacaagttta tggccgtgat gacctgtgtc ttcgtggttt
      781 gtctggtcaa ccaccgcggt ctcagtggtg tacggtacaa acccaaagca gcacgacacg
      841 gcaactacaa gac
//
