LOCUS       sequence-153433-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Supplemental Figure 3. Integration Site 1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP74
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    102..149
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     protein_bind    814..847
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 cgcagagtgt acccacaagc agtaccactg cttcaagtgg tatcgctttg gggaacatgc
       61 cctcggcgcg ggtcttgtag ttgccgtgtc gtgctgcttt gggtttgtac cgtacaccac
      121 tgagaccgcg gtggttgacc agacaaacca cgaagacaca ggtcatcacg gccataaact
      181 tgtggccgtt tacgtcgccg tccagccgaa atagttgttc aattgattga aatagggaca
      241 aataaattaa atttaaagtc tttgggtcag gagaaaccaa aattgggaaa ggtgttcgcc
      301 ttttatattc gattctggtg gtttccaata atctcatgaa aaagaagagg aagaaatcta
      361 aagatgtatt ggcatgatta cataaattcc tacttacgtc ctccgttcat tgatcgtttt
      421 cttcggattc actttgattc tcgtctctgg ccaacctatc ggtccagatt ttctcagcct
      481 ccaagatggt gttattgttg gatctcattt tgttcatggt ttcgatgtac tcgtcatagg
      541 ggtcagaatc gtagacaatt ccacctccgg cttgaaggta agcgacaccg tccttgacga
      601 ccattgttct taaggcaata catgtgtcca tcgattttcc atcgtacgac cagtgtccta
      661 cggcccccgc ataaacacct ctcttttctc cttccaattc tcctatgagt tgcattgctc
      721 ttaccttcgg agcaccggag acggtacctg ctgggaaaat ggatctgaaa gcatcgaagc
      781 gagtcttgtt tggtctcaat gttccacaag cttgaagttc ctattctcta gaaagtatag
      841 gaacttcaga tctgatctca tgcatgacca aaatc
//
